Experimental Cell Biology Flashcards
1
Q
In situ hybridisation ISH
A
-chromosome 5 at metaphase labelled with 6 different DNA probes
2
Q
Hershey and CHase
A
1952
3
Q
In situ hybridisation ISH
A
-chromosome 5 at metaphase labelled with 6 different DNA probes
4
Q
Single stranded RNA molecules
A
-northern blotting
5
Q
Double stranded DNA fragments
A
-southern blotting (must be denatured first)
6
Q
PCR
A
step 1 : heat to separate strands
step 2: hybridisation of primers
steop 3: DNA synthesis from primers
first cycle produces two double-stranded DNA molecules
7
Q
Second cycle
A
produces 4 double-stranded DNA molecules
8
Q
Prophase
A
-replicated chromosomes condense
9
Q
metaphase
A
chromosomes are aligned at the equator of the spindle
-microtubules attach sister chromatids to opposite poles of the spindle
