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Question 1

Taylorella

Answer 1

Reportable dz

Question 2

Taylorella equigenitalis

Answer 2

gram neg

Question 3

Taylorella equigenitalis species

Answer 3

horses
male shows no symptoms, no immunity
mare CEM immunity

Question 4

CEM

Answer 4

contagious equine metritis - STD

only female has symptoms

Question 5

Metritis

Answer 5

asymtomatic carriers highly contagious temporary infertility

Question 6

poor environmental resistance

Answer 6

max 2 days in fridge- will die, care with sampling for dx

Question 7

Taylorella

Answer 7

bacterial sampling of stallion

Question 8

Taylorella equigenitalis sampling of stallion

Answer 8

prepuce
fossa glandis/ sinus urethralis
urethra
RUSH TO LAB- more than 2 days all the other bact will grow Taylorella will die

Question 9

Taylorella equigenitalis sampling mare

Answer 9

sinussen

clitoris

Question 10

Culture Taylorella equigenitalis

Answer 10

capnophilic long incubation

TAKE CARE OF YOUR SAMPLE

Question 11

Dx Taylorella equigenitalis

Answer 11

PCR*

Biochemical testing

Question 12

Prevention Taylorella equigenitalis

Answer 12

eliminate positive animals from breeding, carriers
hard to sanitize
vax poor

Question 13

Taylorella equigenitalis uterine inf

Answer 13

remain carriers
antibiotics not very successful at clearing…will resolve on their own
self limiting

Question 14

Brucella

Answer 14

gram negative
small nonmotile
obligate symbiotic, resistant
obligate pathogen, facultative intracellular***
difficult to culture
REPORTABLE

Question 15

B.melitensis

Answer 15

several animal species mainly goat and sheep

Question 16

b. abortus

Answer 16

cattle

Question 17

b. suis

Answer 17

swine (as well as wildlife)

Question 18

Brucella

Answer 18

High genetic homology- creates problems with dx

Question 19

B.melitensis

Answer 19

sheep and goat

principle cause of human brucellosis

Question 20

B.abortus

Answer 20

many countries have eradicated,

very contagious

Question 21

Brucella serology testing

Answer 21

be aware of y.enterocolitica false positive

Question 22

spread of Brucella

Answer 22

abortion via female, highly contagious. Fetus and placenta contain huge amounts of bacteria

can spread through dogs and horses

Question 23

Brucella abortus

Answer 23

incidence in humans,
high prevalence in Mongolia
eradicated in the USA

Question 24

Brucella abortus pathogenesis

Answer 24

mucosa, oral, wounds
spread to regional lymph nodes then further spread to other LN (intracellular in macrophages for 1-6 wks) No symptoms no immune response, then to target organs (symptoms develop) repro organs, articulations

Question 25

Brucella abortus

Answer 25

calves that survive become carrier | dont drink raw milk in endemic areas

Question 26

Brucella abortus symptoms

Answer 26

intermittent fever 3-4 wks

Question 27

Brucella abortus virulence

Answer 27

cell wall w/ LPS thicker peptidoglycan erythritol- responsible for organ localization ( repro and mamm)

Question 28

smooth colonies

Answer 28

virulence increased more pathogenic and zoonosis not a stable phenotype melitenis,

Question 29

immune rxn Brucella abortus

Answer 29

come late, cellular immunity 1st (abnormal) when immunity goes down- pregnancy- bacteria start multiplication and release from cells, abortion or transfer to fetus

Question 30

vaccination Brucella abortus

Answer 30

need cellular immunity -

Question 31

dx Brucella abortus

Answer 31

slow agglutination rapid agglutination - rose bengal cft ELISA Skin test- swelling rxn because of cytokine production not recommended by OIE. EU regulation only to prove false positive rxns. detect cellular immunity - IFN-gamma produced if cells have been primed ($$$) Bacteriology

Question 32

culture Brucella abortus

Answer 32

grow on specific media needs 10-20% co2 produce h2s primary isolation 4-7d incubation

Question 33

false positives

Answer 33

b. suis y. enterocolitica Tests order 1. saw 2. elisa 3. skin test

Question 34

Brucella suis

Answer 34

pigs

Question 35

Brucella suis

Answer 35

similar to abortus.... resistant in the environment reportable present in wildlife (boars) hunters often infected eradicated in domesticated swine (us cananda eu)

Question 36

Biovars

Answer 36

5 difficult to discriminate most pathogenic 1-3 different reservoirs, hare, reindeer, pigs 5 genetically similar to marine species have been reported in cows, confused with abortus false pos.

Question 37

Brucella suis symptoms

Answer 37

reproduction system , orchitis, infertility, arthritis in PIGS more pathogeneis for humans all general symptoms long term illness fever arthritis

Question 38

Brucella suis pathogenesis

Answer 38

skin oral nasal localization in l. nodes in macrophages, antibodies produced later on organ invation

Question 39

Brucella suis immunity

Answer 39

cellular humoral | carriers in macrophages