Enzyme Kinetics Flashcards
1
Q
Write out Michaelis Constant
A
Km=(K-1+k2)/k1
2
Q
How do you find Km?
A
S when Vmax/2
3
Q
What is Km?
A
Concentration of S which gives 1/2Vmax
4
Q
Competitive Inhibition does what to Vmax and Km?
A
Km varies Vmax doesn’t change
5
Q
Non-competitive Inhibition does what to Vmax and Km?
A
Km stays constant. Vmax varies
6
Q
What is a common mechanism of allosteric control?
A
inhibition of rate limiting enzyme by end products
7
Q
What type of curve do allosteric enzymes produce?
A
sigmoidal curve
8
Q
Give an example of allosteric regulation
A
Haemoglobin
9
Q
A small Km means what?
A
more “efficient” enzyme
10
Q
Draw the Michelins-Menton enzyme substrate thing
A
E+S->//E+P
