3-1 PL Quiz

Question 1

produces an image made from light that is transmitted through a specimen

Answer 1

bright field microscopy

Question 2

what are most biological specimens

Answer 2

transparent

Question 3

how is the transparency of the specimen improved

Answer 3

application of stains to the specimen

Question 4

what is the price of improved contrast of specimen

Answer 4

staining process kills cells

Question 5

how does image formation begin

Answer 5

with light coming from an internal or external light source

Question 6

concentrates the light and makes illumination of the specimen more uniform

Answer 6

condenser lens

Question 7

another name for bending of light

Answer 7

refraction

Question 8

when is the real image produced

Answer 8

during the refraction of light as it passes though the objective lens from the specimen

Question 9

what appears below or within the microscope

Answer 9

virtual image

Question 10

how is the total magnification of the specimen calculated

Answer 10

magnification by objective lens x magnification of ocular lens

Question 11

what is the practical limit to magnification with a light microscope

Answer 11

1300x

Question 12

what is clarity of an image called

Answer 12

resolution

Question 13

another name for limit of resolution

Answer 13

resolving power

Question 14

this is an actual measurement of how far apart two points must be for the microscope to view them as being separate

Answer 14

limit of resolution

Question 15

resolution _______ as the resolution is made ______

Answer 15

improves;smaller

Question 16

the best limit of resolution achieved by a light microscope is

Answer 16

.2 um

Question 17

for a specific microscope the actual limit of resolution can be calculated by using

Answer 17

wavelength/ NA condenser + NA objective

Question 18

the measure of a lens’s ability to capture light coming from the specimen and use it to make the image

Answer 18

numerical aperture

Question 19

using what increases the numerical aperture and makes its limit of resolution smaller

Answer 19

immersion oil

Question 20

a special condenser is used so only the light reflected off the specimen enters the objective

Answer 20

dark field microscopy

Question 21

uses special optical components to exploit subtle differences in the refractive indices of water to produce contrast

Answer 21

phase contrast microscopy

Question 22

light waves that are out of place will produce what

Answer 22

a degree of cancellation or darkness

Question 23

uses a fluorescent dye that emits fluorescence when illuminated with ultraviolet radiation

Answer 23

fluorescence microscopy