Microscopy Flashcards

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1
Q

Resolution

A

How well a microscope distinguishes between two points that are close together

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2
Q

Light microscopes

A

Work by passing light through the specimen

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3
Q

Electron microscopes

A

Use electrons rather than light.

Higher magnification and resolution - can see smaller things in much more detail

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4
Q

Microscope Practical

A
  • take a clean slide and use a pipette to our one drop of water in the middle of it - secure specimen in place, then use tweezers to places you specimen on the slide
  • add stain if specimen is transparent - makes specimen easier to see
  • place cover slip at one end of the specimen, holding it at an angle with a mounted needle and lower it onto the slide. Press it down gently so no air bubbles are trapped. Clip the slide onto the stage.
  • select the lowest powered objective lens
  • use coarse adjustment knob to move stage up so the slide is just underneath the objective lens, looking down at the eyepiece move the stage downwards.
  • adjust the focus with the fine adjustment knob until a clear image. Position a clear ruler on the stage and use it to measure the diameter of the circular area visible
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5
Q

Millimetre x 1000

A

Micrometre (x10-6m)

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6
Q

Micrometre x 1000

A

Nanometre (x10-9m)

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7
Q

Nanometre x 1000

A

Picometre (x10-12m)

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