Microbiology: Listeria Tutorial

Question 1

Via which proteins does listeria gain entry into a cell?

Answer 1

Internalin A and B

Question 2

How does listeria escape the phagolysosome?

Answer 2

LLO (listeria lysin O) production

Question 3

What disease is caused by liisteria that crosses the BBB?

Answer 3

Meningitis

Question 4

How does Listeria avoid non-self recognition?

Answer 4

Encodes ACT-A: this is similar enough in structure to zyxin that the cell mistakes it for zyxin

Question 5

What is the normal function of zyxin?

Answer 5

Involved in focal adhesion formation

Question 6

Where and how do new actin monomers get added onto the comet tail structure of Listeria?

Answer 6

Added to nucleation sites by Arp2/3

Question 7

How is stregth conveyed to the comet tail of listeria?

Answer 7

Cross linking of alpha actinin

Question 8

How is energy provided for actin polymerisation in Listeria infection?

Answer 8

Profilin

Question 9

What is the role of the VASP stabilising complex in Listeria infection?

Answer 9

Facilitates binding of profilin to Arp2/3

Question 10

Where is listeria most commonly found?

Answer 10

Contaminated soil