Gas Chromo.

Question 1

How it separates chemicals

Answer 1

Uses a liquid stationary phase separation is due to the compound’s solubility in the liquid phase.

(high solubility=high retention time)

Question 2

Retention time

Answer 2

time it takes for a compound to travel from the input valve to the detector.

• Retention times can be used to determine a specific compound –> compare RT to a database.

Question 3

Q How could a Relative peak area against conc. graph be obtained in Gas Chromo.

CALLIBRATION!!

Answer 3

• Make up a set of standard solutions with standard concentrations
• Run them through a gas chromatography machine
• Area under each peak is proportional to the known solution. (area under each peak in Adsorption against retention time graph)
• Plot peak area- concentration graph