7.2 - growing microorganisms Flashcards
(15 cards)
What is a petri dish?
A flat, round, disk used to grow and inspect bacteria and other microorganisms.
What is the first step to set up a petri dish?
Clean the petri dish and lid to make it sterile.
What should you mix with water to prepare agar-agar for a petri dish?
10 grams of agar.
How long should you microwave the agar-agar solution?
4 minutes.
After microwaving, how long should you let the agar-agar solution cool?
3 minutes.
What is the next step after pouring the agar-agar solution into the petri dish?
Close it with the lid.
How long should you wait for the agar in the petri dish to solidify?
1 hour.
What is the first step to grow bacteria in a petri dish?
Collect the bacteria and put it on the petri dish.
What are two methods to collect bacteria for the petri dish?
- Leave the petri dish with agar-agar indoors or outdoors for 10 minutes
- Use a cotton ear bud to rub it on a door handle and then on the agar-agar.
What should you do after placing bacteria on the petri dish?
Close the petri dish.
What should you do with the petri dish after closing it?
Turn it the other way round.
What should you use to secure the lid to the petri dish?
Tape.
What should you do after securing the petri dish?
Label it.
Where should you place the petri dish while waiting for the bacteria to grow?
In a dark place.
What safety equipment should you remember to use during these experiments?
- Safety goggles
- Lab coat
- Gloves
