8 - DNA technologies Flashcards
(1 cards)
1
Q
How can we make multiple copies of a particular strand of DNA in vitro?
A
Ingredients: the DNA to be copied, ‘free’ DNA nucleotides, DNA polymerase (thermostable) and primers
Heated to 95ºC for 2-5min breaking hydrogen bonds to produce two DNA polynucleotide template strands
Cooled to 60ºC allowing primers to bind to their target sites free DNA nucleotides complementary base pair with the template strand
Heated to 72ºC - DNA polymerase moves along and joins nucleotides together forming the sugar phosphate backbone.
Two DNA stands have now been produced after one cycle.
