minor procedures

Question 1

What records must be kept by PIL relating to procedures and how long for?

Answer 1

• record of animals procedures carried our
• details of supervision and competence
• retained for 5 years

Question 2

What is PPL standard condition 6?

Answer 2

“For any procedure, the degree of severity imposed shall be the minimum consistent with the attainment of the objectives of the procedure, and this shall not exceed the severity limit attached to the procedure.”

Question 3

When is it acceptable to not carry out a procedure under anaesthesia?

Answer 3

• when anaesthesia is judged more traumatic to the animal than the experiment itself
• anaesthetic is incompatible with the object of the experiment

Question 4

What specific procedures is chemical restraint not necessary for?

Answer 4

• small blood samples
• most injections
• dosing
• collection of urine of faeces
• identification e.g. earclipping

Question 5

Give some examples of procedures where chemical restraint is necessary

Answer 5

• large blood samples
• implants
• hazardous substances
• painful procedures
• dangerous animals

Question 6

What are administration volumes determined by?

Answer 6

• route of administration
• size and species of animal
• should be as small as practical

Question 7

What is the total blood volume (TBV) of mice?

Answer 7

70ml/kg

around 1-1.5ml of blood

Question 8

What are maximal volumes for taking blood?

Answer 8

• no more than 10% TBV at any one time (~150ul)

- No more than 15% TBV in a four week period (~200ul)

Question 9

What blood sampling requires non recovery general anaesthesia?

Answer 9

• cardiac puncture
• abdominal/thoracic blood vessel
• retro-orbital
• decapitation

Question 10

From which vessel may blood sampling require general anaesthesia? (usually for larger volumes)

Answer 10

saphenous vein (leg)
tail vein
sublingual vein
retro-orbital

Question 11

from which vessel does blood sampling require no anaesthesia? (usually smaller volumes)

Answer 11

• saphenous vein (leg)
• tail vein
• manibular vein
• blood vessel canulation
• tail snip (DISCOURAGED)

Question 12

What may be potential animal stressors during blood sampling?

Answer 12

restraint
pain
withdrawel of too much blood
excessive trauma due to poor technique

Question 13

What may be most appropriate peripheral veins for blood sampling in mice?

Answer 13

lateral tail vein
saphenous vein
facial vein (get lots of volume but takes longer to heal)

Question 14

What are the potential adverse effects during blood sampling?

Answer 14

• hypovolaemia
• Haemorrhage
• thrombosis
• phlebitis
• Infection

Question 15

What size needle is appropriate for use on mice?

Answer 15

27G - grey needle