Enzymes 2 Flashcards
1
Q
Enzyme catalyses reaction formula
A
E+S (two way arrow) conc of ES -> P+E
2
Q
How to se how well enzymes are behaving
A
Measuring initial velocity (Vo) using tangent to curve
3
Q
Assumptions of Michaelis-Mentes equation
A
Large number of molecules and small enzyme number
Doesn’t use enzymes that use allosteric regulation
4
Q
What does a low Km mean
A
High substrate affinity
5
Q
How to remove a reversible inhibitor from a system
A
DIalysis
6
Q
What happens when you add an inhibitor
A
Km increases. You need to add more substrate to outcompete inhibitor. No effect on Vmax
7
Q
What’s an uncompetitive inhibitor
A
Binds to enzyme substrate complex only
-and not to the free enzymes
