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BMS242 Cell & Molecular > Yeast Screens (L13?) > Flashcards

Yeast Screens (L13?) Flashcards

1
Q

Enzyme modifications

A

glycosylation and proteolytic cleavage

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2
Q

What are the major membrane trafficking pathways

A

secretory/exocytic pathway, endocytic pathway

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3
Q

What is NOT part of the secretory pathway

A

mitochondria

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4
Q

What does the secretory pathway involve

A

ER to golgi to PM/endosome/lysosome

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5
Q

What does the endocytic pathway involve

A

cell surface to endosome to golgi/ER/lysosom

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6
Q

Protein modification as they transit ER and golgi

A

glycosylated and proteolytically cleaved

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7
Q

Advantage of yeast as model organism for membrane trafficking studies

A

genome sequenced, cheap and easy to grow, large quantities

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8
Q

Disadvantages of yeast as model organism

A

limited cell-cell contact, small, cell wall

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9
Q

Aim of Novick and Schekman experiment in 1980

A

investigate secretory pathway in yeast

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10
Q

Secretory mutants

A

fail to export active invertase and acid phosphatase

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11
Q

Difference in mutant appearance

A

accumulation of vesicles, aberrant membranous structures

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12
Q

How many genes are involved in transport of proteins to membrane

A

23

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13
Q

Where do secreted proteins from class A sec genes accumulate

A

cytosol

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14
Q

Where do secreted proteins from class B sec genes accumulate

A

rough ER

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15
Q

Where do secreted proteins from class C sec genes accumulate

A

ER to golgi transport vesicles

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16
Q

Where do secreted proteins from class D sec genes accumulate

A

golgi

17
Q

What genes did Novick and Schekman miss?

A

genes involved in transport to endosome or vacuole, redundantly functioning genes

18
Q

Where is the decision made to traffic to surface or lysosome

A

TGN

19
Q

What is endocytosis

A

PM invaginates into the cell - vesicle production that fuses with endosome

20
Q

Endocytosis functions

A

down-regulation of signals, remodelling cell surface composition

21
Q

What is the first stage of endocytosis

A

PM to endocytic vesicle

22
Q

What is the last stage of endocytosis

A

late endosome to golgi or vacuole

23
Q

What is important in yeast endocytosis

A

actin cytoskeleton

24
Q

What is the major function of lysosomes

A

degradation of extracellular material

25
Q

What do lysosomes contain

A

degradative/proteolytic enzymes

26
Q

What is carboxypeptidase

A

vacuolar enzyme

27
Q

where is carboxypeptidase transported from

A

ER to golgi

28
Q

How is carboxypeptidase sorted

A

by receptors

29
Q

What does the transport step of sorting require

A

cytoplasmic factors

30
Q

What cytoplasmic factors does the transport step of sorting require

A

clathrin and two adaptors called Gga1 Gga2

BMS242 Cell & Molecular (14 decks)

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