Granulocyte Antigens

Question 1

HNA-1a/1b/1c/1d location

Answer 1

FcÿRIIIb (CD16b)

Question 2

HNA-2 location

Answer 2

CD177

Question 3

antibodies to HNA-2

Answer 3

Made by people missing the whole CD177 protein (1-11%)

Implicated in NAN, TRALI, AIN, and neutropenia in marrow transplant recipients

Question 4

Location of HNA-3a/3b

Answer 4

CTL2 (choline transporter like protein 2)

Question 5

Antibodies to HNA-3a

Answer 5

Most frequent cause of fatal TRALI

Also cause febrile reactions and NAN

Question 6

HNA-4a/4b location

Answer 6

CD11b/18 (plays a role in neutrophil adhesion to endothelial cells and phagocytosis of C3bi opsonized microbes)

Question 7

Location of HNA-5a

Answer 7

CD11a/18

Question 8

Neutrophil expression of ABO and HLA

Answer 8

Do NOT express ABO antigens

Express HLA in modest amounts only upon activation (Class I and II)

Question 9

Antibodies most frequently associated with NAN

Answer 9

HNA-1a, HNA-1b, HNA-2

Question 10

Antibodies most often associated with AIN

Answer 10

HNA-1a (occasionally -1b or -4a)

Question 11

Granulocyte antigen/antibody testing considerations

Answer 11

Granulocytes for testing must be isolated from fresh blood on day of testing (integrity is not maintained in stored samples at any temp)