Lecture 10: mechanisms of catalysis Flashcards
1
Q
transition state = (higher/ lower) energy state
A
higher energy state
2
Q
stabilizing the t state will (inc/ dec) rate of the rxn
A
increase rate
3
Q
2 ways to stabilize t state
A
- protonate carbonyl oxygen making carbonyl carbon more electrophilic [acid catalysis]
- deprotonate H2O making O more nucleophilic [base catalysis]
4
Q
metal ion catalyst
A
electrostatic stabilization of a charged group in the t state
5
Q
when Vo is higher at lower pH, His is
A
protonated [acid catalysis]
6
Q
when Vo is lower at lower pH, His is
A
deprotonated [base catalysis]
7
Q
the pH-rate profile of an enzyme-catalyzed reaction will affect ionization states of
A
all species [enzyme and substrate] in the rate limiting t-state
8
Q
chymotrypsin
A
endoprotease that functions during intestinal digestions; cleaves both proteins and esters
9
Q
the intermediate of chymotrypsin-catalyzed hydrolysis of an ester is
A
between burst and steady phase; not active
