9.6 RBC freezing Flashcards
(28 cards)
RBC freezing is a long term storage for how many years?
10
When RBC is thawed and washed, what is the expiration ?
24 hours
The main purpose of this is to prevent lysis of red cells during freezing?
Cryoprotective agent
2 types of cryoprotective agents
Penetrating and nonpenetrating agent
Type of cyroprotective agent that is a small molecules that cross to the cell membrane into the cytoplasm
Penetrating agent
Example of penetrating agent?
Glycerol
Type of cyroprotective agent that that is a large molecules that do not enter the cell instead, forms a shell around it
Non penetrating agent
Example of nonpenetrating agent?
Hydroxyethyl starch
Dimethylsulfoxide
In high glycerol:
What is the initial freezing temp?
-80C
In low glycerol:
What is the initial freezing temp?
-196C
In high glycerol:
What is the type of freezer?
mechanical
In low glycerol:
What is the type of freezer?
Liquid nitrogen
In high glycerol:
What is the Maximum storage temp?
-65C
In low glycerol:
What is the Maximum storage temp?
-120C
In high glycerol:
What is the Shipping requirements?
Dry ice
In low glycerol:
What is the Shipping requirements?
Liquid nitrogen
In high glycerol:
What is the expiration ?
10 years
Low glycerol is commonly used
T or F
F
High
Process of removing glycerol in the RBC
Deglycerolization
What is the process of deglycerolization?
Decrease saline concentration
Decrease saline is from isotonic to hypotonic
T or F
F
Hyper to iso
What is the 1st decrease saline concentration
12%
What is the 2nd decrease saline concentration
1.6%
What is the 3rd decrease saline concentration
0.09% W/ 0.2% dextrose
