Lecture 5

Question 1

Sanger sequencing is the same as dideoxy chain

Answer 1

True

Question 2

Phosphodiester bonds form between bases

Answer 2

False. Hydrogen

Question 3

Sanger sequencing requires DNA polymerase and single stranded DNA

Answer 3

True

Question 4

Sanger sequencing requires DNA helicase and dNTPs

Answer 4

False. It requires dNTPs but not DNA helicase

Question 5

dNTPs are chain terminators

Answer 5

False. ddNTPS are chain terminators

Question 6

The difference between ddNTP and dNTP is one has hydrogen and one has a hydroxyl group of the 5’ carbon

Answer 6

False. It has these on the 3’ carbon

Question 7

Sanger sequencing used radioactive labels

Answer 7

True

Question 8

Sequences on electrophoresis are read from closest to well to furthest away

Answer 8

False. They are read furthest away (smallest) to closest

Question 9

The minimum fragment size we can use is 10bp is Sanger sequencing

Answer 9

False. It’s 1bp

Question 10

Sanger sequencing can read up to 1000bp

Answer 10

True

Question 11

Next generation sequencing requires a cloning step

Answer 11

False. It doesn’t require a cloning step

Question 12

Next generation sequencing uses ddNTPs

Answer 12

True

Question 13

Next generation sequencing does not have an electrophoresis step

Answer 13

True