Lecture 8

Question 1

CRISPR was discovered in 2013

Answer 1

False. It was discovered in 2000

Question 2

The two repair mechanisms to repair double strand breaks are non-homologous end joining and homologous recombination repair

Answer 2

True

Question 3

In non-homologous end joining, DNA-PK and KU40 are used to repair blunt ends

Answer 3

False. DNA-PK and KU70/80 are

Question 4

Non homologous end joining is mutagenic

Answer 4

True

Question 5

Homologous recombinational repair is normally mutagenic

Answer 5

False. It is not mutagenic but can be through the use of donor molecules

Question 6

Nucleases cut DNA and crease a site specific double strand break

Answer 6

True

Question 7

CRISPR is not a naturally occurring gene

Answer 7

False. It is found in 90% archael genomes and 40% of bacterial genomes

Question 8

CRISPR is present in all genomes

Answer 8

False. It is found in 90% archael genomes and 40% of bacterial genomes

Question 9

CRISPR has a conserved structure of repeat sequences and spacers

Answer 9

True

Question 10

Spacers are always 25 base pairs long

Answer 10

False. They can be between 21-75 base pairs long

Question 11

There are 6 core cas genes

Answer 11

True

Question 12

CRISPR causes double strand breaks

Answer 12

False. Cas genes cause double strand breaks