Lab 1: Determining the Presence or Concentration of Hormones in Body Fluids

Question 1

what is a hormone ?

Answer 1

is a signalling molecule that modulates activity of target tissue

Question 2

what fluids could you use to measure reproductive hormones

Answer 2

milk - things are transferred from blood to milk
blood - serum or plasma
urine- metabolites

Question 3

High progesterone =

Answer 3

cyclic cow in luteal phase or pregnant

Question 4

low progesterone =

Answer 4

in follicular phase, not pregnant or non cyclic

Question 5

What is LFIA?

Answer 5

is a paper based platform for the detection of hormones in body fluids, where the sample is placed on a test device and the results are displayed within minutes

Question 6

LFIA Direct test

Answer 6

the target hormone is immobilized between two complementary antibodies like a sandwich-based assay. thus the presence of the test line indicates a POSITIVE result.

Question 7

LFIA Competitive Test

Answer 7

the hormone blocks the binding sites on the antibodies on the test line, preventing their interactions with the coloured conjugate. therefore, a positive result is indicated by the lack of signal in the test line , control line should be visible regardless of result

Question 8

What are PAGs?

Answer 8

pregnancy associated glycoprotein

they are secreted by the placenta (binucleate trophectoderm cells in ruminants)

Question 9

What is an ELISA?

Answer 9

the ELISA is a plate-based assay technique designed for detecting and quantifying antigens

an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme