Adenoviridae Flashcards
(29 cards)
Genome and replication of Adenoviridae
non-segmented, linear double stranded DNA 35-36kb
Replication in nucleus
virions released by lysis
intranuclear inclusion bodies formed with large number of virions
Adenovirus generalities
agglutinate RBCs
some oncogenic in lab animals
relatively stable in environment but common disinfectants inactivate them
narrow host range
What sort of dz’s do Adenoviridae normally cause?
acute respiratory or gastroenteric dz
mostly subclinical
What proteins do Adenoviruses code for that inhibit class MHC antigen transport?
E3/19K
What proteins does Adenoviridae code for that inhibits tumor necrosis factor induced apoptosis?
E3/14.7K
How do adenoviruses modulate antiviral inflammatory responses?
Inhibit nuclear factor kB transcriptional activity
Where do adenoviruses persist? When are they reactivated? In what sort of animal are they highly pathogenic?
lymphoid tissues
tonsil, adenoids and peyers patches
reactivated in immunocomprimised animals
highly pathogenic in immunodeficient animals
What gene products of Adenoviruses are associated with oncogenesis
E1A: inactivate Rb protein
E1B: inactivate p53 protein
What’s the difference between Mastadenovirus and Aviadenovirus?
mast- single penton fiber
avi- penton fiber is bifurcated, looks like 2 fibers
Infectious Canine Hepatitis (ICH, Rubarth’s Dz)
Etiology
Host
Distribution
Canine adenovirus-1
canidae and ursidae (bears)
worldwide
Infectious Canine Hepatitis
Transmisison
CAV-1 found in all secretions and excretions, shed in urine 6-9 mo
Oronasal transmission
Contact with:
secretions/excretions, fomites, ectoparasites
Morphology of Adenoviridae
nonenveloped hexagonal
icosahedral
720 hexons as 240 trimers
12 vertex penton capsomers w/ a fiber protruding from the surface of the capsid
Why would you see Chronic Glomerulonephritis in a dog with Canine Infectious Hepatitis?
results from immune complex rxns after recovery from acute/subclinical dz
Blue eye is a lesion associated with what viral infection? How many dogs get it? When is it seen?
Infectious Canine Hepatitis/Canine Adenovirus-1
20%
Seen in dogs in recovery or chronic cases
Pathogenesis of Blue Eye
viremia, CAV-1 enters eye via uveal tract
localizes in endothelium of choroid and causes mild uveitis
4-6 days later enter aqueous humor
Day 7 severe anterior uveitis and corneal edema develop due to CAV-1 antibody production and complement activation
Complications from Infectious Canine Hepatitis
DIC
Bacterial pyelonephritis
t/f Icterus is a common symptom of Infectious Canine Hepatitis
False
T/F Corneal edema and anterior uveitis (Blue Eye) occur when clinical recovery begins
True
Dx of Infectious Canine Hepatitis
Clinical Signs
Necropsy- paint brush hemorrhages, liver necrosis, gray-white foci in kidney cortex
Dx of ICH
Biochemistry and hematology- leukopenia, increased ALT and AST, proteinuria, thrombocytopenia Virus isolated in urine FAT PCR Paired serum testing ELISA of HI
What is the preferential vx for CAV-1?
CAV-2 live attenuated
Kennel Cough
Etiology
Transmission
CAV-2 and Bordetella bronchiseptica
highly contagious, aerosolized droplets
Canine Infectious Tracheobronchitis/Kennel Cough
Clinical Signs
Uncomplicated ITB vs Complicated
Incubation period 1-3 days
Uncomplicated- dry honking cough, rhinitis, nasal dc
Complated- severe pnemonia or bronchopneumonia, life threatening
Dx of ITB/Kennel Cough
clinical signs, palpating larynx or trachea
PCR
