AHG Test

Question 1

Also knowns COOMBS Test

Answer 1

Antihuman Globulin Test

Question 2

detects non-agglutinating antibody by means of COUPLING WITH SECONDARY ANTIBODY

Answer 2

AHG TEST

Question 3

It bridges the distance between cell that IgG alone cannot do

Answer 3

Antihuman globulin

Question 4

• In VIVO attachment
• Uses RED CELL as specimen

Answer 4

Direct AHG Test

Question 5

Anticoagulant used in DAT

Answer 5

EDTA

Question 6

What is preferred EDTA in DAT pink OR violet

Answer 6

PINK

Question 7

AHG TEST USED IN:
1. Investigation of HTR
2. Investigation of HDN
3. Investigation of AIHA
4. Diagnosis of Drug-induced

Answer 7

DAT

Question 8

• Non-agglutinating antibody
• sensitized and coat with Red cells
• without causing agglutination

Answer 8

IgG

Question 9

Drug-Induced Hemolytic Anemia:

Drug Absorption

Answer 9

Penicillin

Question 10

Drug-Induced Hemolytic Anemia:

Membrane modification

Answer 10

Cephalosphorin

Question 11

Drug-Induced Hemolytic Anemia:

Immune-complex

Answer 11

PaRa Sa Immune-complex

Phenacetin
Rifampin
Stibufen
Quinidine

Question 12

Drug-Induced Hemolytic Anemia:

Autoantibody formation

Answer 12

Methyldopa (Aldomet)
Mefenamic acid

Question 13

Increased OFT ; Increased MCHC

are both observed in Hereditary Spherocytosis and AIHA. What is the test needed to differentiate the two?

Answer 13

DAT

Hereditary Spherocytosis (-)
AIHA (+)

Question 14

AHG Reagent specifically binds on the __ portion of IgG

Answer 14

Fc portion

Question 15

• Determines the presence of PARTICULAR ANTIBODY in patient
• In VITRO sensitization
• uses SERUM/PLASMA spx

Answer 15

IAT
(Indirect AHG Test)

Question 16

AHG TEST used in:
1. Crossmatching
2. Antibody detection
3. Antibody Identification
4. RBC Phenotyping

Answer 16

IAT

Question 17

Anticoagulant used for IAT

Answer 17

NONE
Red top tube

Question 18

2 PROCESS OF IAT

Answer 18

1. Washed RBC and Antibody are allowed to combine at 37 deg cel
2. Washed RBC again 3x and add AHG Test

Question 19

Washing of RBC is require to

Answer 19

remove unbound antibody

Question 20

Presence of unbound antibody effect

Answer 20

neutralizes AHG Reagent

Question 21

AHG reagent color

Answer 21

green

Question 22

• consist of anti-IgG AND anti-c3d
• obtained from RABBITS

Answer 22

Polyspecific AHG

Question 23

• consist of anti-IgG OR anti-c3d
• obtained from MICE

Answer 23

Monospecific AHG

Question 24

If (+) for Monoclonal AHG,sample is subject for

Answer 24

Elution

Question 25

Patient with AIHA coats the patient cells with warm IgG antibody , thus requires

Answer 25

Removal of antibody from RBC for accurate RBC phenotyping

Question 26

Techniques used to Remove Antibody from RBC

Answer 26

1. Chloroquine 2. EDTA-glycine 3. Murine monoclonal antibodies

Question 27

- used to validate NEGATIVE RESULT OF AHG TEST - uses Group O sensitized with IgG

Answer 27

Check O cells

Question 28

Check O cells (+) agglutination means:

Answer 28

1. AHG was added 2. AHG was not neutralized If (-) check cells then INVALID TEST

Question 29

1. contamination of reagents 2. Overcentrifugation 3. Direct agglutination of strong agglutinin 4. Over-incubation 5. Improper use of enhancement reagents 5. Saline stored in glass or metal containers

Answer 29

False POSITIVE (+) AHG

Question 30

1. Reagent failure 2. Failure to add AHG reagent 3. Improper WASHING 4. Improper centrifugation 5. Serum/Cell ratio TOO LOW 6. Delay in washing

Answer 30

False NEGATIVE (-) AHG

Question 31

Elution of weakly attached Antibody is due to

Answer 31

Delay in washing

Question 32

Enhancement Reagent for AHG (4)

Answer 32

1. Albumin 2. Polybrene 3. Low Ionic Strength Solution (LISS) 4. Polyethylene Glycol (PEG)

Question 33

Enhancement Reagents that shortens the incubation time for 10-15 mins

Answer 33

LISS PEG