Albumin Measurement

Question 1

Measurement principle:

Answer 1

Albumin + BCG ———-> Albumin- BCG complex

Question 2

most common method for its detection involves the use of a ______

Answer 2

dye Bromcresol Green (BCG)

Question 3

reference method for detection involves the dye of _________

Answer 3

Bromcresol Purple (BCP)

Question 4

Albumin normal values:

Newborn (0-14 days):
Children (4-14 days):
14 - 18 yrs:
Adults;

Answer 4

Newborn (0-14 days): 2.8 - 4.4
Children (4-14 days): 3.8 - 5.4
14 - 18 yrs: 3.2 - 4.5
Adults; 3.4 - 4.8

Question 5

Mix and incubate for _____ at 25C or _____ at 37C. Measure the absorbance against reagent blank.

Answer 5

10 mins

5 mins

Question 6

Computation

Answer 6

g/dL = 4 x SA sample/SA standard

Question 7

Conversion factor:

Answer 7

g/dL to g/L = 10

g/L to g/dL = 0.1

Question 8

Albumin present in the sample is made to react with ___, forming the _______. The complex produces _____ color. The more intense the color is, the more albumin is present

Answer 8

BCG
albumin-BCG complex.
blue-green

Question 9

Assay procedure

Wavelength:
Temperature:

Answer 9

Wavelength: 578 nm
Temperature: 25-37C