Total Bilirubin Flashcards
1
Q
No fasting is required
A
2
Q
Measurement principle:
A
Van den Berg Reaction
3
Q
Reagents
Reagent A:
Reagent B:
Reagent C:
A
Reagent A:
Dicholoroaniline
HC1
Detergent
Reagent B:
Sodium Nitrite
Reagent C (Rgt blank);
Dicholoroaniline
HC1
Detergent
4
Q
Assay Procedure
Wavelength:
Temperature:
A
Wavelength: 405 nm
Temperature 37C
5
Q
Mix reagent with ___ratio. Before use, allow to reach room temperature for at least ______ (dark storage)
A
1:1
15 minutes
6
Q
Mix and allow to rest for _____ (dark storage). Measure the absorbance of the sample (A sample) against sample blank (A blank) within _____.
A
10 minutes
30 minutes
7
Q
Computation
A
SAs = A sample - A sample blank
mg/dL = SAs x 12.5
8
Q
Reference Values:
A
0.8 - 2.0 mg/dL
