Amniotic Fluid Flashcards

1
Q

What is the unit used when talking about pregnancy?

A

Weeks

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2
Q

What weeks are there in the 1st trimester?

A

1st to 12th week

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3
Q

Weeks that the 3rd trimester represent

A

27 weeks to day of delivery

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4
Q

Main purposes of the amniotic fluid

A
  • To cushion and protect the fetus
  • Enables movement of the fetus
  • Provide nutrients
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5
Q

The amniotic fluid fills a membrane called ______.

A

Amnion

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6
Q

The membrane that the amniotic fluid fills up is composed of what type of cells?

A

Cuboidal epithelial cells

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7
Q

In the late stages of pregnancy, how is the fetus contributing to the contents of the amniotic fluid?

A

The fetus has already developed lungs and kidneys, thus it can already swallow some fluid and then urinate.

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8
Q

True or false:

The fetus is able to inhale the amniotic fluid

A

True

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9
Q

What is the most commonly performed method of collection of the amniotic fluid?

A

Transabdominal amniocentesis

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10
Q

The amniotic fluid is typically collected after ____ weeks of gestation.

A

14

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11
Q

In the collection of amniotic fluid, why is the first 2-3ml discarded?

A

To avoid contamination

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12
Q

At how many weeks of gestation do we assess for neural tube defects or genetic abnormalities?

A

14-18 weeks

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13
Q

At how many weeks of gestation do we assess for the pulmonary and fetal health status?

A

20-42 weeks

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14
Q

Modified true or false:

Amniotic fluid has urea and creatinine levels equal to the urine

A

False

Plasma

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15
Q

In cases of fetal death, what is the color of the amniotic fluid?

A

Dark-red brown

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16
Q

This test is done in cases of anticipated premature deliveries.

A

Fetal lung maturity test

17
Q

This is measured to indicate the maturity of the lungs of the fetus

A

Surfactants

18
Q

Surfactants are made out of:

A

90% phospholipids, 10% protein

19
Q

Surfactants are packaged into storage granules called ________.

A

Lamellar bodies

20
Q

Lamellar bodies are secred by:

A

Type 2 pneumocytes

21
Q

What are some of the tests that determine the fetus’ lung maturity

A

Lecithin/Sphingomyelin ratio
Phosphatidylglycerol
Foam stability index
Lamellar body count
Flourescence polarization assay

22
Q

Lecithin/sphingomyelin ratio is tested using _____.

A

Thin layer chromatography (TLC)

23
Q

Amniostat-FLM uses what antibodies?

24
Q

How do you report the result for AmnioStat?

A

negative, low positive, & high positive

25
Also known as the shake test
Foam stability test
26
Modified true or false: The foam stability test is a confirmatory test for the lung maturity of the fetus.
False Screening test
27
In foam stability test, the amniotic fluid is mixed with _____ then shaked for ____ seconds.
95% ethanol, 5 seconds
28
Amniotic fluid bilirubin is tested using what method?
Spectrophotometry
29
What is the oldest routinely performed test on AF?
Amniotic fluid bilirubin testing
30
The AF bilirubin optical density is measured in intervals between ____ nm and ____ nm.
365, 550
31
At what wavelength can bilirubin be absorbed?
450nm
32
Neural tube defects may be dected by measuring _______.
Maternal serum alpha-fetoprotein
33
Examplea of NTDs
Anencephaly Spina bifida