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Bacteriology 2 > Anaerobes > Flashcards

Anaerobes Flashcards

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1
Q

Organisms that does not require oxygen for growth

A

Anaerobes

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2
Q

What are the four (4) macroscopic exam done to identify anaerobes?

A
  1. Presence of foul odor
  2. Presence of blackening/black color of specimen
  3. Fluorescence
  4. Presence of sulfur granules
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3
Q

Three (3) anaerobes that emit foul odor

A
  1. Clostridium difficile
  2. Fusobacterium
  3. Porphyromonas
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4
Q

Equipment used to provide an anaerobic environment

A

GASPAK Jar

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5
Q

Indicators of GASPAK Jar

A

Methylene Blue or Resazurin

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6
Q

Component of GASPAK jar that ensures oxygen free
environment

A

Palladium Catalyst

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7
Q

What happens when there’s a presence of oxygen in GASPAK jar?

A

The jar turns red or blue depending on the indicator that is present.

Methyl Blue (Blue)
Resazurin (Pink)

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8
Q

Absence of color in the GASPAK jar indicates what?

A

Absence of air

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9
Q

Common failure of GASPAK jar

A

Inactivation of catalyst due to repeated use

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10
Q

Gram negative anaerobic bacilli that produce black pigment and brick red fluorescence under UV

A

Porphyromonas and Prevotella

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11
Q

Anaerobes that produces red fluorescence that rapidly fades

A

Veilonella

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12
Q

The color of fluorescence Fusobacterium nucleatum emits

A

Chartreuse fluorescence (Yellow)

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13
Q

Three (3) anaerobes with sulfur granules

A
  1. Actinomyces,
  2. Propionibacterium/Cutibacterium acnes
  3. Eubacterium nodatum
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14
Q

Anaerobic organism that is microscopically with tapered ends appearing like toothpick

A

Fusobacterium nucleatum

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14
Q

Anaerobic organism with “bifid” ends/appearing like bone-shaped dog biscuit

A

Bifidobacterium

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15
Q

Anaerobic bacilli that assumes safety pin appearance

A

Bacteroides fragilis

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16
Q

Bacteroides fragilis shows resistance to which antibiotics in Potency Disk Test?

A
  1. Kanamycin
  2. Vancomycin
  3. Colistin
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17
Q

Bacteroides fragilis is bile tolerant.

a. True
b. False

A

a. True

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18
Q

Anaerobic bacilli that causes aspiration pneumonia

Study These Flashcards
A

Bacteroides fragilis

19
Q

Anaerobic diphtheroid

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A

Propionibacterium spp.

20
Q

This test is done for rapid detection of Cutibacterium acnes

Study These Flashcards
A

Spot Indole Test

21
Q

What is the positive result for spot indole test?

Study These Flashcards
A

Development of blue/green color

22
Q

What is the negative result for spot indole test?

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A

Pink/orange color

23
Q

The reagent used in Spot Indole Test

Study These Flashcards
A

P-dimethylaminocinnamaldehyde

24
Match: Spot Indole Test A. Cutibacterium acnes B. Propionibacterium spp. 1. Negative (-) 2. Positive (+) a. Pink/red b. Blue/green
Cutibacterium acnes - Positive - Blue/Green Propionibacterium spp. - Negative - Pink/Red
25
The purpose of this test is to verify gram staining result of anaerobic bacteria
Potency Disk Test
26
Antibiotics used in Potency Disk Test and their concentration
1. Kanamycin (1000 µg) 2. Vancomycin (5 µg) 3. Colistin (10 µg)
27
Identify the bacteria: Result in Potency Disk Test: RRR
Bacteroides fragilis
28
Identify the bacteria: Result in Potency Disk Test: SRS
1. Fusobacterium 2. Bacteroides ureolyticus 3. Veilonella
29
Identify the bacteria: Result in Potency Disk Test: SSR
Clostridium
30
Identify the bacteria: Result in Potency Disk Test: RSR
1. Porphyromonas 2. P. anaerobius
31
Identify the bacteria: Result in Potency Disk Test: RRS
Prevotella
32
Anticoagulant used in bacterial culture and its concentration
Sodium Polyanethol Sulfonate (0.25%)
33
Disadvantage of SPS
Peptostreptococcus anaerobius is sensitive.
34
How do you counteract the inhibitory property of SPS?
Add 1% gelatin
35
Spore-forming anaerobes that exhibit swarming on BAP
1. Clostridium tetani 2. Clostridium septicum
36
Anaerobic organism that develops colonies appearing like molar tooth
Actinomyces israelii
37
Actinomyces israelii affects soft tissues and can cause?
Actinomycosis of Jaw (Lumpy Jaw)
38
Anaerobic staphylococcus
Peptococcus niger
39
Anaerobic streptococcus
Peptostreptococcus
40
Anaerobic organism that is an important component of dental biofilm
Prevotella
41
Sign of growth in Thioglycolate media
Turbidity
42
A versatile media an often used in the lab because it can allow growth of anaerobe, facultative anaerobes and aerobes
Thioglycolate media
43
Sign of growth of anaerobes on Thioglycolate media
Turbidity at the bottom of the media
44
Sign of growth of facultative anaerobes on Thioglycolate media
Diffused growth all throughout the media
45
Sign of growth of strict aerobes on Thioglycolate media
Turbidity towards the surface

Bacteriology 2 (9 decks)

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