Fermenters

Question 1

Media that is used to detect fermentation/acid production with Bromthymol blue as indicator

Answer 1

OF Media of Hugh and Leifson

Question 2

Media that is used to detect fermentation/acid production with Phenol red as indicator

Answer 2

OF Media - CDC method

Question 3

How many tubes are used in OF Media of Hugh and Leifson?

Answer 3

Two (2) tubes

Open (aerobic) and closed (anaerobic)

Question 4

OF Media of Hugh and Leifson:

1. Positive result:
2. Negative result:

a. Yellow
b. Blue green/Green

Answer 4

1. Positive result – a. Yellow
2. Negative result – b. Blue green/Green

Question 5

Interpretation of result: OF Media of Hugh and Leifson

Open/Closed

1. +/+
2. +/-
3. -/-

a. Oxidizer/Non-fermenters
b. Non-oxidizers/Non-saccharolytic
c. Fermenter

Answer 5

1. +/+ = Fermenter
2. +/- = Oxidizer/Non-fermenters
3. -/- = Non-oxidizers/Non-saccharolytic

Question 6

How is Triple Sugar Iron dispensed?

Answer 6

As slant & butt

Question 7

Oxygen requirement of Enterobacteriaceae

Answer 7

Facultative anaerobe

Question 8

All Enterobacteriaceae are non-fermenters.

a. True
b. False

Answer 8

b. False.

All are fermenters.

Question 9

Usual media to detect Enterobacteriaceae’s ability to produce acid (ferment)

Answer 9

Triple Sugar Iron

Question 9

What are the three (3) fermentable carbohydrates in TSI and their corresponding measurement?

Answer 9

1. Glucose (1 part)
2. Lactose (10 parts)
3. Sucrose (10 parts)

Question 10

TSI media can also be used as H2S indicators.

a. True
b. False

Answer 10

a. True

Question 11

How can TSI show H2S production?

Answer 11

TSI has two (2) H2S indicators: Ferrous sulfate and sodium thiosulfate

Question 12

Match: TSI

1. Acid pH
2. Alkaline pH

a. K
b. A

Slant/Butt color:

• Yellow
• Red

Answer 12

1. Acid pH - A - Yellow
2. Alkaline pH - K - Red

Question 13

Manner of reporting of TSI result

Answer 13

Slant/Butt

Question 14

Match the results:

1. K/K
2. K/A
3. A/A

a. Non-lactose fermenter
b. Lactose fermenter
c. Non fermenter

Slant/Butt Color:

• Yellow
• Red

Answer 14

1. K/K - Non fermenter - Red/Red
2. K/A - Non-lactose fermenter - Red/Yellow
3. A/A - Lactose fermenter - Yellow/Yellow

Question 15

E.coli, Enterobacter, Klebsiella are what type of fermenters?

Answer 15

Lactose fermenters

Question 16

Shigella spp., Proteus, Providencia and Morganella are what type of fermenters?

Answer 16

Non-lactose fermenters

Question 17

Citrobacter, Y. enterocolitica, and S. sonnei are what type of fermenters?

Answer 17

Late lactose fermenters

Question 18

P. aeruginosa is what kind of fermenter?

Answer 18

Non-fermenter

Question 19

Two possible results of Enterobacteriaceae in TSI

Answer 19

1. K/A
2. A/A

Question 20

Four (4) tests that will differentiate Salmonella-Shigella

Answer 20

1. Motility test
2. H2S production
3. Malonate
4. Decarboxylase test

Question 21

Salmonella or Shigella:

• Motile, H2S (+), Malonate and Decarboxylase (+)

Answer 21

Salmonella

Question 22

Salmonella or Shigella:

Nonmotile, H2S (-), Malonate and Decarboxylase (-)

Answer 22

Shigella

Question 23

Salmonella and Shigella are what kind of pathogens?

a. Respiratory
b. Oral
c. Genital
d. Intestinal

Answer 23

d. Intestinal pathogens

Both are agents of diarrheal disease

Question 24

Salmonella and shigella are never normal flora. a. True b. False

Answer 24

a. True

Question 25

These characteristics will separates Vibrio spp. from the Genus Aeromonas.

Answer 25

Vibrio spp. - String test (+); Susceptible to Vibriostatic Agent Aeromonas - String test (-); Resistant to Vibriostatic Agent

Question 26

Vibriostatic agent is also known as

Answer 26

0/129 agent

Question 27

Selective differential media for Vibrios

Answer 27

Thiosulfate Citrate Bile salt Media (TCBS)

Question 28

pH Indicator of TCBS

Answer 28

Thymol blue Bromthymol blue

Question 29

What is the carbohydrate present in TCBS?

Answer 29

Sucrose

Question 30

Color of colonies that are sucrose fermenters

Answer 30

Yellow

Question 31

Color of colonies that are non-sucrose fermenters

Answer 31

Green

Question 32

Enrichment media for Vibrios

Answer 32

Alkaline Peptone Water

Question 33

Vibrio spp. that are non-sucrose fermenters

Answer 33

V. mimicus, V. parahaemolyticus, V. vulnificus (MPV)

Question 34

Vibrio spp. that are sucrose fermenters

Answer 34

V. alginolyticus V. cholerae, V. fluvialis V. furnissi V. metschnikovii (ACFFM)

Question 35

All Vibrios are halophilic, except:

Answer 35

1. V. cholerae 2. V. mimicus Both are non-halophilic

Question 36

How much salt concentration is required for Vibrios?

Answer 36

8-10%

Question 37

Other term for Beta-hemolysis due to TDH

Answer 37

Kanagawa phenomenon

Question 38

Media used to demonstrate Kanagawa phenomenon of V. parahaemolyticus

Answer 38

Wagatsuma agar

Question 39

Component of Wagatsuma agar

Answer 39

High salt mannitol concentration

Question 40

Causative agent of Summer Diarrhea in Japan

Answer 40

V. parahaemolyticus

Question 41

Causative agent of Rice watery stool

Answer 41

V. cholerae

Question 42

Cholera is what kind of disease, and characterized by?

Answer 42

Diarrheal disease characterized by Rice watery stool

Question 43

What is TDH?

Answer 43

Thermostable Direct Hemolysis

Question 44

Characteristic of Vibrio that will separate them from Enterobacteriaceae

Answer 44

Vibrio - Oxidase (+) Enterobacteriaceae - Oxidase (-)

Question 45

Characteristic of Vibrio that will separate them from Plesiomonas

Answer 45

Vibrio - Inositol (-) Plesiomonas - Inositol (+)

Question 46

Characteristic of Vibrio that will differentiate them from Pseudomonas

Answer 46

Vibrio - Fermenters (all glucose fermenters; lactose (-) except V. vulnificus Pseudomonas - Non-fermenters

Question 47

Vibrio spp. that is less virulent, occupational hazard → continuous contact with sea water (fishermen, sailor)

Answer 47

V. alginolyticus

Question 48

Most significant vibrio

Answer 48

V. cholerae

Question 49

Species that is Triple Decarboxylase negative (-)

Answer 49

Pantoea agglomerans

Question 50

Pantoea agglomerans is negative to which amino acids?

Answer 50

Negative to Lysine, Ornithine and Arginine

Question 51

Species positive in Triple Decarboxylase

Answer 51

Pleisomonas spp.

Question 52

This test is done to detect if the organism produces decarboxylase enzyme

Answer 52

Decarboxylase test

Question 53

What are the two (2) ways to do Decarboxylase test?

Answer 53

1. Moeller's broth 2. LIA

Question 54

Carbohydrate and indicator present in Moeller's broth

Answer 54

Glucose Bromcresol purple

Question 55

Color result in Decarboxylase test

Answer 55

(+) purple (-) yellow

Question 56

What does decarboxylase enzyme do in decarboxylase test?

Answer 56

Decarboxylase enzyme removes carboxyl group from the amino acid

Question 57

Match: Decarboxylase Test Amino acids 1. Lysine 2. Ornithine 3. Arginine Carboxyl Group a. Putrescine b. Citrulline c. Cadaverine

Answer 57

1. Lysine - Cadaverine 2. Ornithine - Putrescine 3. Arginine - Citrulline

Question 58

Campylobacter spp. that appears S-shaped or wings of seagulls

Answer 58

C. jejuni and C. coli

Question 59

Motility of C. jejuni and C. coli

Answer 59

Darting motility

Question 60

Growth requirement of Campylobacter jejuni and Campylobacter coli

Answer 60

1. Capnophilic 2. Microaerophilic

Question 61

The virulence factor of which organism is production of urease?

Answer 61

Helicobacter pylori

Question 62

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Answer 62

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