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Analysis of cell components Flashcards

(6 cards)

1
Q

Magnification

A
  • size of image / size of real object

- how much bigger the image is than the specimen

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2
Q

resolution

A
  • how detailed image is

- how well a microscope distinguishes between 2 points that are close together

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3
Q

oprical (light) microscope

A
  • light to form an image
  • max res 0.3 micrometers
  • cant use to view smaller organelles than 0.2
  • max magnification x1500
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4
Q

electron microscope

A
  • use electrons to from an image
  • higher res so give more detailed image
  • max res is 0.0002 micrometers
  • max magnification x 1500000
  • produces black and white image but can be coloured by a computer
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5
Q

transmission electron microscopes

A
  • use electromagnets to focus a beam of electrons so transmitted through the specimen
  • denser parts absorb more electrons so makes them look darker on the image
  • higher res image so you see internal structures of organelles
  • view specimen in a vacuum no good for living organisms
  • only on thin specimens
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6
Q

Scanning electron microscopes

A
  • can beam of electrons across the specimen
  • knock off electrons from specimen gathered in a cathode ray tube to form an image
  • images show surface of specimen and be 3D
  • used on thick specimens
  • lower res the TEMs
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