Analysis of cell components Flashcards
1
Q
Magnification
A
- size of image / size of real object
- how much bigger the image is than the specimen
2
Q
resolution
A
- how detailed image is
- how well a microscope distinguishes between 2 points that are close together
3
Q
oprical (light) microscope
A
- light to form an image
- max res 0.3 micrometers
- cant use to view smaller organelles than 0.2
- max magnification x1500
4
Q
electron microscope
A
- use electrons to from an image
- higher res so give more detailed image
- max res is 0.0002 micrometers
- max magnification x 1500000
- produces black and white image but can be coloured by a computer
5
Q
transmission electron microscopes
A
- use electromagnets to focus a beam of electrons so transmitted through the specimen
- denser parts absorb more electrons so makes them look darker on the image
- higher res image so you see internal structures of organelles
- view specimen in a vacuum no good for living organisms
- only on thin specimens
6
Q
Scanning electron microscopes
A
- can beam of electrons across the specimen
- knock off electrons from specimen gathered in a cathode ray tube to form an image
- images show surface of specimen and be 3D
- used on thick specimens
- lower res the TEMs