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Flashcards in Analytical Chemistry Deck (6)
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1

Define: mobile phase

The solvent in the chromatography process, which moves through the column or over the paper/thin layer

2

Define: stationary phase

The immobile phase in chromatography that the mobile phase passes over or through.

3

Paper chromatography:
-stationary phase
-mobile phase
-process of separation

Mobile phase: solvent
Stationary phase: water trapped between cellulose fibres of paper
Process: partition, calculate Rf value

4

Thin layer chromatography:
-mobile phase
-stationary phase
-process of separation

Mobile phase: solvent
Stationary phase: thin solid layer which adsorbs solid molecules onto its surface (alumina or silica supported on inert base e.g. glass)
Process: adsorption (partitioning can occur if solid becomes rehydrated)

5

HPLC
-moblie phase
-stationary phase
-process of separation

Mobile phase: high-purity solvent (usually polar, forced under pressure through column)
Stationary phase: non-volatile liquid bonded onto a solid support (column)
Process: partitioning, record retention time

6

GLC
-mobile phase
-stationary phase
-process of separation

Mobile phase: inert carrier gas
Stationary phase: