Antibody

Question 1

What does FAB and Fc means?

Answer 1

FAB arms - bind to antigen. Fragment antigen binding

Fc - effector function. Fragment crystallisable

Question 2

Name the shapes the antibody epitope can be

Answer 2

Pockets
Grooves
Extended surfaces
Projections on antigen binding site

Question 3

What genes encode the VH domain?

Answer 3

V, D and J genes

V - variable
D - diversity
J - joining

Question 4

What genes encode for the light chain?

Answer 4

V and J genes

V - variable
J - joining

Question 5

At what stages do rearrangements occur?

Answer 5

Early pro-B : D-J rearranging

Late pro-B : V-DJ rearranging

Small pre-B : V-J rearranging

Question 6

Name the stages of B cell development

Answer 6

Stem cell -> Early Pro B -> Late Pro B ->

Large Pre B -> small pre B -> Immature B -> Mature B

Question 7

What is the 12/23 rule

Answer 7

Only a pair of dissimilar spacer RSSs are efficiently recombined

Question 8

What enzyme aids class switching?

Answer 8

Activation induced cytidine deaminase (AID)

Only found in proliferating B cells

Question 9

What is allelic exclusion?

Answer 9

Each B cell produces a Ig of single antigen specificity. Only one light chain and one heavy chain are expressed

Question 10

How are antibodies classed?

Answer 10

By their heavy chain