Artificial Recombinants (Bacteria)

Question 1

RNase

Answer 1

Ribonuclease (Full name), Is an enzyme that is used to break down the exciting structures of RNA (RiboNucleic Acid). This is widely used in operation of plasmid mini-prep.

Question 2

DNase

Answer 2

q

Question 3

Transformation (Bacterial Recombination)

Answer 3

q

Question 4

Competent Cells

Answer 4

q

Question 5

Electroporation

Answer 5

q

Question 6

Replica Plating

Answer 6

q

Question 7

Insertional Inactivation

Answer 7

q

Question 8

Direct Selection

Answer 8

q

Question 9

Vector (Bacterial Recombination)

Answer 9

q

Question 10

‘Unique’ Restriction Site

Answer 10

q

Question 11

Library Screening

Answer 11

q

Question 12

The Griffiths & Avery experiment

Answer 12

q

Question 13

Selectable Marker Genes

Answer 13

q

Question 14

Origin of Replication

Answer 14

q

Question 15

What are the three important characteristics of pUC plasmids?

Answer 15

q

Question 16

Direct selection

Answer 16

q

Question 17

Genomic Library

Answer 17

q

Question 18

pUC Plasmid

Answer 18

q

Question 19

LacZ

Answer 19

q (X-gel)

Question 20

What are the befits of library screening?

Answer 20

q

Question 21

Plasmid Purification (What is it and why do we do it?)

Answer 21

q

Question 22

What are the different steps of ‘plasmid mini preps’?

Answer 22

q

Question 23

CaCl2+HeatShock Therapy

Answer 23

q

Question 24

Restriction Enzymes

Answer 24

q

Question 25

Palindrome

Answer 25

q

Question 26

Antiparallel cleavage

Answer 26

q (Sticky end)

Question 27

Parallel cleavage

Answer 27

q (Blunt end)

Question 28

Dimeric Enzymes

Answer 28

q

Question 29

Genetic palindromes can very in length, what is the standard length of bases in a palindrome and what are the common extents of them?

Answer 29

q (C6-base palin, can range from 4-8 base palin though)

Question 30

What befits are there to cleaving a restriction site in an asymmetrical faction?

Answer 30

q (The base sequence can be exposed and we can get allot of stick end reassosiactions based on the natural hydrogen bonds that can accrue between complementary base pairs.

Question 31

DNA Ligase

Answer 31

q (Is unspecific to what it joins up so can fix foreign DNA or even connect different fragments that the original cleave site. This can result in gene linkage, and can be a process where my we can control where genes are located on a chromosome.) Can join any DNA with compatible sticky end.

Question 32

Recombinant DNA

Answer 32

q

Question 33

What are the benefits of recombinant DNA?

Answer 33

(New bacteria with new useful properties, and can allow a natural protein synthesis factory.)

Question 34

Genome

Answer 34

q

Question 35

PCR

Answer 35

q

Question 36

Plasmids (What are they, where do they come from, and what are their function?)

Answer 36

(accessory Chromosomes)

Question 37

How can we clone the gene of interest in organic systems?

Answer 37

q