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Bact211 > AUTOCLAVING > Flashcards

AUTOCLAVING Flashcards

1
Q

What is the principle of autoclave?

A

Heat under steam pressure

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2
Q

It is at this temperature in which all microorganisms and their endospore are destroyed within approximately 15 minutes

A

Autoclave

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3
Q

What is the recommended temperature for media, liquids, utensils, glass pipettes?

A

121•C or 15 psi for 15 minutes

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4
Q

What is the recommended temperature for decontaminating medical wastes?

A

132•C or 15 psi for 30-60 minutes

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5
Q

The main component of a steam autoclave, it is where the materials to be sterilized are placed

A

Pressure chamber

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6
Q

The purpose of this is to seal the chamber and maintain the sterilizing conditions within the autoclave

A

Lid

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7
Q

It indicates the pressure inside the autoclave

A

Pressure gauge

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8
Q

Pressure within the autoclave can be controlled by lifting the whistle and releasing certain amount of vapor

A

Pressure releasing unit

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9
Q

Present underneath the chamber to heat the water and generate steam

A

Steam generator

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10
Q

Give precautions when using autoclave

A
  • Wastes and clean items should be autoclaved separately
  • do not attempt to ooen the lid when the autoclave is running
  • avoid placing materials that might melt inside the autoclave
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11
Q

Involves the direct application of high heat in materials such as inoculating loops.

A

Direct flaming

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12
Q

What are the two types of direct flaming?

A

Open flame and incinerator

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13
Q

Creates free radicals that causes intracellular damage

A

Radiation

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14
Q

Causes mutation in the DNA; destroys vegetative cells and endospore

A

Ionizing radiation

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15
Q

Damages cellular DNA

A

Non-ionizing radiation

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16
Q

Utulizes ultraviolet rays

A

Non-ionizing radiation

17
Q

Utilizes gamma rays and xrays

A

Ionizing radiation

18
Q

Used to pasteurize meat products

A

Ionizing radiation

19
Q

Used to exposed surfaves and rooms

A

Non-ionizing radiation

20
Q

First widely used antiseptic and disinfectant; destroys plasma membranes and denature cell proteins

A

Phenols

21
Q

Used at 1:10 dilution ; 3 minutes contact time

A

Chlorine (hypochlorite)

22
Q

Denatures proteins and causes dissolution of lipid membranes ; effective at 60-90% concentration

A

Alcohol

23
Q

What is the dilution formula?

A

C1V1=C2V2

Bact211 (10 decks)

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