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Bact211 > VIBRIO > Flashcards

VIBRIO Flashcards

1
Q

Vibrio is commonly found in

A

Fresh water, salt water, and estuarine water

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2
Q

How can you avoid vibrio infection?

A

Avoid eating raw or undercooked shellfish particularly in warmer summer months

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3
Q

What are the most commonly isolated vibrio species?

A
  • vibrio cholerae
  • vibrio parahaemolyticus
  • vibrio vulnificus
  • vibrio alginolyticus
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4
Q

Causes a devastating diarrheal disease cholera

A

Vibrio cholerae

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5
Q

Causes ulcers and has been linked to gastric carcinoma

A

Helicobacter pylori

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6
Q

What are the factors to consider when identifying vibrio infection

A
  • recent consumption of raw seafood
  • recent immigration travel
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7
Q

What species of vibro are catalase positive and oxidase negative?

A

Vibrio metschnikovii

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8
Q

What is the causative agent for cholera?

A

Vibrio cholerae O1

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9
Q

Vibrio cholerae O1 also known as

A

Asiatic cholera

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10
Q

Most common cause of bacterial gastroenteritis

A

Campylobacter jejuni

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11
Q

Aeromonas wound infection almost always present as

A

Cellulitis

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12
Q

A test that is used to differentiate vibrio from members of enterobacteriaceae

A

Oxidase test

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13
Q

Campylobacter fetus is associated with

A

Bacteremia

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14
Q

What is the culture medium of choice for isolation of vibrio species?

A

Thiosulfate Citrate Bile Salt Sucrose

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15
Q

Sucrose ferments in TCBS appears as

A

Yellow

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16
Q

What is the pH indicator in TCBS

A

Bromothymol blue

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17
Q

What is the reagent for string test?

A

Sodium desoxycholate

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18
Q

Selective agar for pseudomonas aeruginosa

A

Cetrimide agar

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19
Q

The concentration of the vibriostatic disc

A

150 ug

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20
Q

What is the atmospheric environment requirement for campylobacter jejuni and what is the incubation temperature?

A

5% oxygen and 10% carbon dioxide at 42 degrees

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21
Q

What causes the formation of mucoid string in string test?

A

DNA

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22
Q

Acute diarrheal disease spread through contaminated water

A

Cholera

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23
Q

Cholera is responsible to what toxin?

A

Cholera toxin or choleragen

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24
Q

What are the two biovar of cholera?

A

Classical type and el tor type

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25
Q

What type of cholera tested positive in voges proskauer?

A

El tor

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26
Q

El Tor is susceptible in which susceptibility testing?

A

Polymyxin B susceptibility test

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27
Q

How do we replace fluids lost from the severe diarrhea?

A

Oral fluids or intravenous

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28
Q

What is the treatment for cholera?

A

Azithromycin or ciprofloxacin

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29
Q

Cholera is resistant to

A

Tetracycline and doxycycline

30
Q

What is the second most common vibrio implicated in gastroenteritis?

A

Vibrio parahaemolyticus

31
Q

Vibrio parahaemolyticus is first recognized in which country?

A

Japan

32
Q

Heat stable hemolysin that is able to lyse human erythrocytes

A

Kanagawa toxin

33
Q

Special high salt mannitol medium

A

Wagatsuma agar

34
Q

It is least pathogenic and most frequently isolated vibrio

A

Vibrio alginolyticus

35
Q

What are the two infections of vibrio vulnificus?

A
  • primary septicemia
  • wound infection
36
Q

Transport media for vibrio

A

Cary Blair media

37
Q

What is the color of non sucrose fermenting for vibrio

A

Green colonies

38
Q

For enrichment procedure what medium is used?

A

Alkaline peptone water with 1% sodium chloride

39
Q

What is the most commonly isolated specie of aeromonas?

A

A. caviae

40
Q

Septicemia, meningitid, and wound infections

A

Extraintestinal infections

41
Q

What is the pigment for aeromonas colonies?

A

Translucent and white to buff-colored

42
Q

What test is used to differentiate plesiomonas and aeromonas?

A

Fermentation of inositol

43
Q

What specie of aeromonas is lactose fermenter?

A

A. caviae

44
Q

What is the mode of transmission for C. Jenuni?

A
  • direct contact with animals and handling infected pets
  • consumption of water and dairy products
  • person to person transmission
45
Q

What is the atmospheric environment requirement for helicobacter pylori and what is the incubation temperature?

A

5-10% oxygen and 5-12% carbon dioxide at 37 degrees

46
Q

What genus plays a role in GBS or Guillan-Barre Syndrome?

A

Campylobacter spp

47
Q

This genus is classified as carcinogen

A

Helicobacter spp

48
Q

What is the specimen for c. jejuni?

A

Stool

49
Q

What is the specimen for c. fetus?

A

Blood with incubation at 35-37 degrees

50
Q

What is the transport medium for campylobacter?

A

Cary-Blair medium

51
Q

What is the transport medium for Helicobacter?

A

Stuart medium

52
Q

What is the specimen for Helicobacter?

A

Gastric biopsy material

53
Q

What is the selective media for c. jejuni?

A

Campy-BAP

54
Q

What other media used in c. Jejuni?

A

Skirrow’s agar and butzler medium

55
Q

What is the commonly used medium in Helicobacter pylori?

A

CAP or brucella agar with 5% horse blood

56
Q

What is the selective medium for h. pylori?

A

Skirrow’s agar

57
Q

What is the shape for campylobacter?

A

Seagull-wing shaped

58
Q

What is the shape for vibrio?

A

Comma shaped

59
Q

What is the shape for Helicobacter?

A

Curved shaped or U shaped

60
Q

What is the recommended counter stain for campylobacter?

A

Carbolfuschin

61
Q

What does campylobacter looks like under phase contrast microscope?

A

Darting motility or corkscrew movement

62
Q

What is the colony morphology c. fetus?

A

Smooth, convex, translucent colonies

63
Q

What is the colony morphology for c. jejuni?

A

Moist, runny looking, and spreading

64
Q

What biochemical test is used to differentiate Helicobacter from campylobacter?

A

Urease test

65
Q

What test is used to distinguish aeromonas from vibrio?

A

String test and vibriostatic O/129

Aeromonas
String test negative
Vibriostatic O/129 resistant

Vibrio
String test positive
Vibriostatic O/129 susceptible

66
Q

Toxin choleragen consists of

A

Two toxic a subunits and five binding b subunits

67
Q

What are the three test used to differentiate el tor and classical cholera?

A

Voges proskauer test
Polymyxin B susceptibility test
Serological test using Chicken RBC as antigen

68
Q

What is the colony morphology of vibrio in SBA?

A

Medium to large colony ; smoith, opaque, iridescent with greenish hue

69
Q

It is found in soil and aquatic environments
Does not tolerate increased sodium chloride

A

Plesiomonas

70
Q

Widely distributed among warm and cold blooded animals like dogs, cats, lizard, fish, shellfish

A

Plesiomonas

71
Q

What is the mode of transmission for plesiomonas?

A

Ingestion of contaminated water or food

72
Q

What is CIN?

A

Cefsulodin-irgasan novobiocin

Bact211 (10 decks)

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