Automation of Machines Flashcards

1
Q

Photons travelling in waves

A

Electromagnetic radiation

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2
Q

Distance between two peaks

A

Wavelength

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3
Q

Distance between peak and trough

A

Amplitude

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4
Q

Falls in between 400 nm to 700 nm

A

Visible lights

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5
Q

The concentration of a substance is directly proportional to the amount light absorbed or inversely proportional to the logarithm of the transmitted light

A

Beer lawbert law (Beers law)

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6
Q

Method of determining the concentration of substance in solutions; measuring amount of light absorbed by that solution

A

Spectrophotometer

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7
Q

Most common light source in visible and near infrared regions

A

Incandescent tungsten or tungsten iodide lamp

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8
Q

Most common used for UV work

A

Discharge lamp and mercury arc lamp

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9
Q

Will select your desired wavelength

A

Monochromators

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10
Q

Most common used in monochromators

A

Diffraction grating

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11
Q

Round vs square cuvette

A

Preferred square cuvette

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12
Q

Used for application in the visible range or infrared

A

Glass

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13
Q

For application requiring UV radiation

A

Quartz

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14
Q

Layer cell or photo cell; least expensive

A

Barrier

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15
Q

Used in instrument designed to be extremely sensitive to very low light level and light flash

A

Photomultiplier tube

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16
Q

Measures the quantity of light reflected by a liquid sample that has been dispensed onto a grainy or fibrous solid support

A

Reflectometry

17
Q

Measurement of concentration is done by detecting absorption electromagnetic by atom rather than molecules

A

Atomic absorption spectrophotometry

18
Q

Usual light source of AAS

A

Hallow cathode lamp

19
Q

Break chemical bond and form free unexcited atoms serves as sample cells (AAS)

20
Q

Used to isolate the desired wavelength (AAS)

A

Monochromators

21
Q

Measurement of light emitted by excited atoms

A

Flame Photometry

22
Q

Measurement of the concentration of the solutions that contain flourescing molecules; used to measure small particles

A

Flurometry

23
Q

Used for protein blotting, immunoassay, pharmacological and toxicological testing

A

Chemiluminescence

24
Q

Measurements made with spectrophotometer to determine a concentration of particulate matter in sample

A

Turbidimetry

25
Light scattered by a small particles is measured at an angle to the beam incident to the cuvette
Nephelometry
26
Measure particles which are too larger for spectrophotometry
Nephelometers
27
Measurement of the current or voltage generated by the activity of spiecific ion
Electrochemistry
28
Measurement of potential voltage between two electrode in a solute to measure analyte concentration
Potentiometry
29
Measurement of the current flow produced by an oxidation reduction reaction
Amperometry
30
Electrochemical titration in which titrant is electrochemically generated
Coulometry
31
Potential is applied to an electrochemical cell and the resulting current is measured
Voltammetry
32
Separation of charged compounds based on their electric charge
Electrophoresis