B- forms and function Flashcards
all the long questions (4+) (73 cards)
What are the basic building blocks of life?
Molecules
Molecules form the foundation of cells, which in turn make up organisms.
What is the smallest unit of life?
Cells
Cells are the basic structural and functional units of all living organisms.
What is the term for a community of interacting organisms and their environment?
Ecosystem
Ecosystems consist of living organisms and their physical environment.
What process involves the copying of DNA?
DNA replication
DNA replication is essential for cell division and the continuity of genetic information.
What enzyme unwinds and unzips the DNA during replication?
DNA helicase
DNA helicase breaks hydrogen bonds to form the replication fork.
What is the role of DNA polymerase III?
Synthesizes new DNA strands
DNA polymerase III adds nucleotides in a 5’ to 3’ direction.
How does DNA polymerase I function in DNA replication?
Removes RNA primers and replaces them with DNA nucleotides
This enzyme ensures that the newly synthesized DNA is continuous.
What is the purpose of the polymerase chain reaction (PCR)?
Amplifies small fragments of DNA
PCR is crucial for advancements in medical and forensic science.
What are the stages of PCR?
Denaturation, Annealing, Extension
These stages involve heating DNA, binding primers, and replicating DNA.
What is gel electrophoresis used for?
Separating DNA fragments based on size and charge
This technique allows visualization of DNA fragments after amplification.
What is the primary product of transcription?
Messenger RNA (mRNA)
mRNA carries genetic information for protein synthesis.
Where does transcription occur in eukaryotic cells?
Nucleus
In prokaryotic cells, transcription occurs in the cytoplasm.
What is the role of transcription factors?
Bind to promoters to regulate transcription
They can increase or decrease RNA polymerase activity.
What happens during the elongation phase of transcription?
RNA nucleotides find complementary bases on the DNA
RNA polymerase catalyzes the formation of covalent bonds between nucleotides.
What is alternative splicing?
Allows one gene to code for multiple proteins
This process can remove additional exons from pre-mRNA.
What is the function of tRNA?
Brings amino acids to the ribosome
tRNA has an anticodon that is complementary to mRNA codons.
What is the start codon for translation?
AUG
This codon signals the beginning of protein synthesis.
What is the role of ribosomes in translation?
Facilitate the synthesis of proteins
Ribosomes consist of large and small subunits that coordinate tRNA and mRNA.
What are the three binding sites on a ribosome?
A, P, E sites
A site holds the tRNA carrying the next amino acid; P site holds the growing polypeptide; E site is where tRNA exits.
What is a mutation?
Permanent structural changes to genes
Mutations can affect protein function and may be beneficial, neutral, or harmful.
What are single-nucleotide polymorphisms (SNPs)?
Base substitutions in DNA sequence
SNPs can be synonymous (no change in amino acid) or non-synonymous (change in amino acid).
What is the CRISPR-Cas9 system used for?
Gene editing
This system allows for precise modifications of DNA sequences.
What is the function of cyclins in the cell cycle?
Regulate the progression of the cell cycle
Cyclins activate enzymes that phosphorylate target proteins.
What is the outcome of mitosis?
Production of diploid daughter cells
Mitosis results in cells that are genetically identical to the parent cell.