bacte euclid Flashcards
(321 cards)
transfer of genetic material from donor bacterial strain to recipient strain in a form of plasmid
conjugation
transfer of bacterial genes by a bacteriophage from one cell to another
transduction
it refers to uptake and incorporation of naked DNA into a bacterial cell
transformation
of bacterial morphology
4
unit of measurement for bacteria
micrometer (micron)
these are sites of respiratory activity of bacteria
mesosome
unique component of the outer membrane of gram-negative bacteria
lipopolysaccharide
they grow in the absence of O2 and obtain O2 from O2-containing compounds
anaerobes
term used to denote “cold-loving” bacteria
psychrophile/cryophile
what media are made of extracts of meat or soybean (ex. nutrient broth, trypticase soy broth)
nutrient media
proper manner of dispensing culture media (4)
weigh agar
dissolve agar in flask
sterilize
dispense into petri dish
to dissolve the agar powder, pour half the volume of _ in a vessel, then the _, the pour the rest of the _
water, agar, water
type of water used in preparing culture media
deionized or distilled water
how to determine if an agar is completely dissolved
it should be clear (no particles)
purpose of peptone in culture media
source of organic nitrogen
phenylethyl alcohol is added on certain media to _
inhibit gram negative bacteria
why should Thioglycolate broth be boiled for 10 mins before being used
to driven off oxygen
in what broth, organisms will grow only in the part of broth where O2 concentration meets their needs
Thioglycolate broth
obligate aerobes grow _ of THIO broth (O2 is 20-21%)
at the top
microaerophiles grow _ of the THIO broth where the oxygen is around 5%
near the top
obligate anaerobes grow _ of THIO broth (O2 is 0%)
at the bottom
facultative anaerobes and _ anaerobes grow _ THIO broth
aerotolerant, in the entire
it is added to culture media tubes to provide and maintain an anaerobic environment
mineral oil
composition of blood agar
nutrient agar + 5% sheep blood