Bacteria short info

Question 1

Listeria monocytogenes:

Answer 1

§ Haemolysis +
§ Rhamnose +
§ Xylose –
§ CAMP test: S.aureus (+), Rh.equi (-)

Question 2

Listeria ivanovii:

Answer 2

§ Haemolysis +
§ Rhamnose –
§ Xylose +
§ CAMP test: S.aureus (-), Rh.equi (+)

Question 3

Salmonella:

Answer 3

§ BPLS agar: purple-red colonies
§ Cannot decompose lactose and sucrose
§ BPL agar: purple colonies
§ XLD agar: reddish purple colonies
§ Decomposes glucose
§ H2S production +
§ Urease test –
§ Indole test +(Salmonella is Indole NEGATIV)
§ Voges-Proskauer –
§ Methyl red +
§ ONPG-reaction –

Question 4

Enterobacteriaceae:

Answer 4

§ EE broth: yellowish with turbidity, green on the bottom
§ VRBG agar: pink to red colonies, with or without precipitation halos, or colorless
§ Oxidase negative
§ Gram negative
§ Glucose fermentative
§ Reduce nitrate to nitrite

Question 5

E.coli:

Answer 5

§ Citrate utilization test -
§ Voges-Proskauer test -
§ Methyl red test +
§ Indole test +