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Bacterial Cultivation Flashcards

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1
Q

Refers to the increase in the number of bacteria rather than in size.

A

BACTERIAL GROWTH

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2
Q

True or False

In bacteria, growth refers to the number, not in size

A

TRUE

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3
Q

Bacterial growth requirements

A

NUTRITIONAL & ENVIRONMENTAL

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4
Q

Salt-loving bacteria

A

HALOPHILIC/HALOPHILES

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5
Q

Halophilic bacteria

A

Staphylococcus spp.
Enterococcus spp.
Vibrio spp. except Vibrio cholerae and Vibrio mimicus
Bacillus spp.

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6
Q

Bacteria that are difficult to grow

A

FASTIDIOUS

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7
Q

Absolutely requires oxygen to grow l, live, and survive

A

STRICT/OBLIGATE AEROBE

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8
Q

Strict/Obligate Aerobe

A

Micrococcus spp.
Mycobacterium spp.
Pseudomonas spp.
Neiserria spp.
Brucella spp.
Francisella spp.
Bordatella spp.
Leptosprira spp.

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9
Q

Bacteria that have the ability to grow, live, and survive in small concentration of oxygen environment( hindi maarte)

A

FACULTATIVE ANAEROBE

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10
Q

Facultative Anaerobe

A

Staphylococcus spp.
Streptococcus spp.
Family of Enterobacteriaceae

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11
Q

Bacteria that prefers small concentration of oxygen environment approximately 2%-10%

A

MICROAEROPHILIC

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12
Q

Microaerophilic

A

Campylobacter spp.
Helicobacter spp.
Arcobacter spp.
Streptococcus spp.

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13
Q

Absolutely do not require oxygen to grow, live, and survive.

A

STRICT/OBLIGATE ANAEROBE

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14
Q

Strict/Obligate Anaerobe

A

Many Clostridium spp., such as Clostridium noyvi, most Bacteroides spp., Fusobacterium spp., Peptostreptococcus spp., and Porphyromonas spp.

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15
Q

Bacteria that do not require oxygen but may tolerate or withstand limited exposure to oxygen.

A

AEROTOLERANT ANAEROBE

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16
Q

Aerotolerant Anaerobe

A

Some Clostridium spp., such as Clostridium perfringens, Bacteroides fragilis, most strains of Proprionibacterium and Lactobacillus

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17
Q

Bacteria that requires 5%-10% CO2 to grow

A

CAPNOPHILIC/ CAPNOPHILES/
CAPNOPHILIC BACTERI

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18
Q

Capnophilic Bacteria

A

(N) Neisseria spp. (C) Cardiobacterium spp.
(H) Haemophilus spp. (E) Eikenella spp.
(A) Aggregatibacter spp. (K) Kingella spp.

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19
Q

Optimally growing between 20-40˚C

A

MESOPHILIC

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20
Q

Growing optimally between 0-20˚C

A

PSYCHROPHILIC

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21
Q

50-60˚C

A

THERMOPHILIC

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22
Q

80-110˚C & Spore-forming bacteria

A

HYPERTHERMOPHILIC

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23
Q

Wide range of temperature

A

EURITHERMOPHILIC

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24
Q

Wide range of temperature

25
Acid loving bacteria
ACIDOPHILIC
26
Alkali loving bacteria
ALKALIPHILIC
27
High osmotic pressure
OSMOPHILIC BACTERIA
28
The bacteria are adjusting to the environment Cellular activity: synthesis of proteins & DNA is already happening. No cell division is happening * The cell activities are needed for the cell division
LAG PHASE
29
* From the lag phase, the logarithmic suddenly rises; the bacteria are increasing; rapid generation of bacteria. * Growth rate: The number of generation per hour * The phase where bacteria is the most metabolically active; susceptible to antimicrobial agents * There is cell division = increase in number
LOGARITHMIC GROWTH PHASE
30
* The nutrients are running out; toxins are produced that are harmful to microorganisms * Rate of cell division = Rate of cell deaths
STATIONARY OR PLATEU PHASE
31
Bacterial growth no longer occurs; more dying than viable
DEATH (DECLINE PHASE)
32
Mimicking the environment and nutrition that is originally the reservoir
CULTURE MEDIA
33
Composed of known and exact amounts of pure chemical substances; commonly used for culturing autotrophic microorganisms
SYNTHETIC/CHEMIMCALLY DEFINED
34
* Composed of complex materials that are rich in vitamins and nutrients that are not usually represented by a chemical formula such as peptones, beef or yeast extract, plant extracts etc. * Combination of chemicals and complex materials that are rich in minerals and vitamins
NON-SYNTHETIC/NON-CHEMICALLY DEFINED/ COMPLEX
35
* Live cells harvested from organs of humans and animals that supports the growth of obligate intracellular organisms that cannot grow in artificially prepared culture media. * Commonly used for viruses
TISSUE CULTURE MEDIA
36
a culture medium that doesn’t contain a solidifying agent; dissolved in water
LIQUID
37
* A culture medium that contains 0.5% - 1% agar * Solidifying agent: agar (most common) (it is not easily degraded by bacteria; it can be melted and can be resolidify upon cooling: 42˚C then melt upon boiling) * Sulfide Indole Motility (SIM) Medium: Used for observation of hydrogen sulfide gas production, indole production & motility * What is often desired to be observed is motility
SEMI-SOLID
38
A culture medium which contains 1.5% - 3%T (2%-3% in other books) agar
SOLID
39
Usually contained in a container that can be made of glass (pyrex) or disposable plastic (standard: 100 mm in terms of diameter) (AST: 150mm)
PLATED
40
Usually, container in glass tubes such as Wassermann tubes with different volume capacity (3mL, 5mL, 10mL) or in a tube with a flat bottom and a screw cap
TUBED
41
Culture media contained in a glass bottle that is usually used for blood culture
BOTTLED
42
* Contains basic nutritional requirements to support the growth of non-fastidious microorganisms * This is also used a base medium in the preparation of other culture media
GENERAL PURPOSE/ PRIMARY/ BASIC/ BASAL/ SUPPORTIVE/ GENERAL ISOLATION CULTURE MEDIA
43
Contains the basic nutritional requirement to support the growth of non-fastidious microorganisms with additives (special requirements: blood), enriching substances, or supplements to support the growth of fastidious microorganisms. Ex: BAP, CAP
ENRICHED CULTURE MEDIA
44
A primary media used to support or favor the selective growth of pathogens in a specimen, such as stool or sputum, where the number of normal flora outnumber the pathogens
ENRICHMENT BROTH
45
Favors the growth of the organism of interest using inhibitors added in the culture media
SELECTIVE CULTURE MEDIA
46
Contains indicators which changes in color as a result of a product produced be a chemical reaction in the components of the media such as glucose
DIFFERENTIAL CULTURE MEDIA
47
True or False Not all selective culture media are differential but all differential culture media are selective
TRUE
48
A primary isolation culture media which maintains the viability of bacteria allowing rapid multiplication if there is an anticipated delay in bringing the specimen collected bedside or remotely to the laboratory
TRANSPORT CULTURE MEDIA
49
Stuart’s Transport Medium
Neisseria spp.
50
Cary-Blair
Vibrio spp.
51
Amie’s
Neisseria spp. (with charcoal)
52
Transgrow, JEMBEK
brand
53
JEMBEK
Jon E. Martin Biological, Environmental, Chamber
54
Used to selectively favor the growth of Vibrio while inhibiting all other normal intestinal flora due its high Ph
Alkaline Peptone Water (APW)
55
Used to selectively favor the growth of Salmonella while inhibiting all other normal intestinal flora
Selenite F Broth
56
An enrichment broth for anaerobic bacteria but should be used solely in the isolation of anaerobic bacteria since it can also grow aerobes and facultative anaerobes
Thioglycolate Broth (THIO)
57
Used to selectively favor the growth of Salmonella and Shigella while inhibiting all other normal intestinal flora
GN Broth (Gram Negative Broth)
58
A liquid enrichment recommended for the production of Streptococcal haemolysin (virulence factor of Streptococcus) and the cultivation of Streptococci prior to serological grouping
Todd-Hewitt Broth
59

Bacteriology (2 decks)

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