bacterial genetics Flashcards

(55 cards)

1
Q

long double stranded supercoiled circular DNA

A

bacterial chromosomes

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2
Q

extra genetic information, small circular DNA

A

plasmids

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3
Q

the chromosomes subdivided into

A

genes

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4
Q

what is essential for bacterial growth ?

A

genes

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5
Q

the genes that has specialized function are carried on

A

plasmids

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6
Q

Function of Rifamipcin

A

Inhibit RNA ploymerase

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7
Q

macrolides aminoglycosides tetracyclcin chloramphenicol
drugs that..

A

inhibitor of protein synthesis

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8
Q

how does the plasmids replicate ?

A

autonomously
they replicate independently of replication of the bacterial chromosomes

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9
Q

Plamsids with high copy number are replicate under

A

relaxed control of replication

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10
Q

plasmids with low copy number their replication is

A
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11
Q

the plasmids carry some genetic information which are

A

not essential for growth

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12
Q

plasmids can transfer from cell to cell so they are used as?

A

vectors

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13
Q

Plasmids are used as vetors for what

A

cloning DNA

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14
Q

types of plasmids

A

conjugative
non conjugative

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15
Q

charcaterastics of conjugative plasmids

A

large
copy number 1-2
present f factors
transfer among bacteria conjugation

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16
Q

what bacteria contain conjugative plasmids ?

A

gram negative bacilli

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17
Q

how can conjugative plasmids transfer among bacteria

A

conjugation

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18
Q

contain tra gene and
sex pilus formation

A

conjugative plasmids

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19
Q

stringent

A

conjugative plasmids

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20
Q

characterstics of non conjugative plasmids

A

small
copy number 10-60
absent f factor

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21
Q

transfer among bacteria by help of conjugative plasmids

A

non conjugative plasmids

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22
Q

what plasmids can be seen in gram positive cocci

A

non conjugative

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23
Q

does not contain tra gene nor
sex plius formation

A

non conjugative plasmids

24
Q

what is the gene that resist drug in ?

A

R-Factors plasmids

25
the f factors is seen in what plasmids
conjugative
26
sex factors plasmids are carried on
f factor
27
fonction of f factor
mediate gene transfer by conjugation
28
Rabid spread of drug resistance
conjugation
29
bacterial properties on plasmids are
fertility drug resistance virulence production of antimicrobial production of bacteriocins
30
large groups of genes that cause pathogenicity located in chromosomes
pathogeneicity islands
31
what are pathogeneicity islands size
10 to 200 kbp
32
changes in bacterial characters due to underlying genetic changes
genotype
33
changes in bacterial charcters under influence of the environment with no change genetic compustion
phenotype
34
mutation gene transfer 1- transformation 2- conjugation 3- transduction are examples of what /
examples for genotypic variations
35
permanent and heritable
genotypic
36
non heritable and reversible
phenotyoic
37
change s in bases sequence of DNA result in insertion of different amino acids
mutation
38
the resultant of mutation
changing in protein product if the affected gene heritable and irreversable
39
x rays ,ultraviolet, and alkyl agents
mutagenic agents
40
molecular changes that causes mutations
base substitution insertion deletion frames shift mutation
41
recombination of 2 genetically different DNA from bacterial (recipient) to cell donor
gene transfer
42
gene transfer methods
transformation transduction conjugation
43
what are method of transformation
in nature dying bacteria release their DNA which are may be taken up by another bacteria - in laboratry by exteracted DNA and introduced genetically to different bacteria by CaCl and heat shook
44
fragments of DNA transfered to another bacterium by bacteriophage
transduction
45
types of generalized transduction
lytic phages
46
in what cycle the generalized transduction occur
lytic
47
the mechanism of generalized transduction is.
Error in assembly
48
the genes in generalized transduction
any gene chromosomal or plasmids
49
in what cycle specialized transduction
temperate lysogenic the one that the bacterophage on
50
in what cycle specialized transduction occur
lysogenic
51
what is the mechanism of specialized transduction
error in excision
52
what is the genes associated with specialized transduction
chromosomal genes next to site of insertion of prophage
53
meeting of 2 bacterial cells of the same or different species the DNA transferred from a donor to recipient
conjugation
54
what cause insertion mutation
transposon
55
define transposons
non replicating gene that move readily within or between the DNAs of bacteria ,plasmids ,and bacteriohage