Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Microbiology and Parasitology (Lecture) > Bacteriology > Flashcards

Bacteriology Flashcards

(63 cards)

Start Studying
1
Q

a subdivision of microbiology which involves the identification, classification, and characterization of bacterial species.

A

Bacteriology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

why was the diminutive “bakteria” used in the latinization of the word bacterium?

A

because bakteria means “staff, cane” and the first discovered bacteria were rod-shaped

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what type of microorganisms do bacteria constitute?

A

a large domain of prokaryotic microorganisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what do single-celled bacteria fail to have around their nucleus or organelles because of their prokaryotic nature?

A

membrane enclosure (and membrane enclosed organelles like mitochondria and chloroplasts)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

(based on growth requirements)
do not grow on ordinary culture media, requiring additional growth factors for successful cultivation

A

fastidious microorganism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

(based on growth requirements)
are easily cultivated because of their ability to grow on ordinary media such as NA and NB

A

non-fastidious microoganisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

(based on growth on media)
state all types of media

A
  • non-selective media (usually enriched medium)
  • differential media
  • selective media
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

(based on growth on media)
suppresses growth of unwanted bacteria and encourage growth of desired ones

A

selective media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

(based on growth on media)
list well known selective media

A
  • Bismuth Sulfite Agar (BSA)
  • Potato/Sabouraud’s Dextrose Agar
  • Loeffler Medium
  • Tellurite Media
  • Bordet-Gengou Agar
  • Thayer-Martin Agar Plates
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

(selective media)
what cultivates mostly Salmonella species (S. typhi) from feces

A

Bismuth Sulfite Agar (BSA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

(selective media)
what does BSA inhibit?

A

gram positive and most gram negative intestinal bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

(selective media)
what is used to cultivate fungi, yeasts, and molds from foodstuffs

A

Potato/Sabouraud’s Dextrose Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

(selective media)
what is Potato//Sabouraud’s Dextrose Agar’s pH?

A

5.6

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

(selective media)
what is used to cultivate Corynebacterium diptheriae?

A

Loeffler Medium and Tellurite Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

(selective media)
what is used to cultivate Bordetella pertussis?

A

Bordet-Gengou Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

(selective media)
what chocolate agar is used to cultivate Neisseria gonorrhoeae?

A

Thayer-Martin Agar Plates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

(selective media)
why do Thayer-Martin Agar Plates contain antibiotics?

A

in order to suppress normal flora from mixed bacteria and fungi (it is both selective and enrichment)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

(selective media)
what is used to cultivate Legionella?

A

Charcoal Yeast Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

(based on growth on media)
distinguish colonies of the desired organism from other colonies

A

differential media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

(differential media)
based on pattern of hemolysis

A

Blood Agar Plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

(differential media)
Blood Agar Plate that is incomplete, light greenish

A

alpha-hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

(differential media)
Blood Agar Plate that is complete, light transparent

A

beta-hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

(differential media)
Blood Agar Plate that has no reaction

A

gamma-hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

(based on growth on media)
MSA and Mannitol

A

Selective and Differential Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
(selective and differential media) what is used to cultivate Staphylococcus aureus?
Mannitol Salt Agar
25
(selective and differential media) what makes MSA selective?
7.5% NaCl
26
(selective and differential media) differential for fermenters and non-fermenters
mannitol
27
(selective and differential media) fermentation changes color of phenol red via
red to yellow
28
round or oval bacterial shape
cocci
29
rod shaped bacteria
bacilli
30
twisted or coiled shaped bacteria
spirals
31
curved rod shaped bacteria
vibrio
32
bacteria that do not have a definite shape
pleomorphic
33
developed by Hans Christian Gram
Differential Gram Stain
34
steps for gram staining
-Crystal Violet -Iodine -Alcohol -Safranin
35
disperse in nigrosine or india ink -> spread using another slide -> gently heat -> stain w/ crystal violet/safranin -to distinguish capsular material from the bacterial cell
capsular staining
36
results of capsular staining
halo appearance (purple/pink cell + dark background + colorless capsule)
37
positive for capsule staining (Some Killers Have Pretty Nice Capsules)
- Streptococcus pneumoniae - Klebsiella pneumoniae - Haemophilus influenzae - Pseudomonas aeruginosa - Neisseria meningitidis - Cryptococcus neoformans (fungi)
38
binds to waxy material in cell walls and resists decolorization with acid alcohol
acid-fast stain
39
acid fast bacteria (May Nuno)
- Mycobacterium - Nocardia
40
steps for acid fast staining (CHAM)
Ziehl-Neelsen Method - Crystal Violet - Heat - Alcohol - Methylene blue
41
spore forming bacteria (BaCcla)
- Bacillus - Clostridium
42
results of spore staining?
green endospore w/n red or pink cells
43
steps for spore staining (MHWS)
Schaeffer-Fulton Method - Malachite green - Heat - Water - Safranin
44
ALL COCCI are gram-positive, except NVM
- Neisseria - Veillonella - Moraxella
45
require O2 for survival
obligate aerobes
46
bacteria that are obligate aerobes (PutaNginaBabyMoCya)
- Pseudomonas - Nocardia - Bacillus - Mycobacterium - Corynebacterium
47
grow only in the absence of O2
obligate anaerobes
48
bacteria that are obligate anaerobes (ABCEFP)
- Actinomyces - Bacteroides - Clostridium - Eubacterium - Fusobacterium - Prevotella
49
microorganism that requires environments containing lower levels of dioxygen than that are present in the atmosphere -require ↓ O2, however ↑ O2 concentration -> toxic)
microaerophiles
50
microaerophilic bacteria (CollegeHumor)
- Campylobacter - Helicobacter
51
tolerates O2 but cannot replicate in its presence
aerotolerant
52
aerotolerant bacteria (LaPilluz)
- Lactobacillus - Propionibacterium
53
used to distinguish organisms on the basis of the presence or absence of a certain respiratory enzyme, in which the family Enterobacteriaceae can be differentiated from other gram-negative rods
oxidase test
54
what respiratory enzyme is often absent in the Enterobacteriaceae family?
cytochrome C
55
this test is particularly useful for the differentiation of gram positive cocci
catalase test
56
what cocci can be differentiated from the catalase test?
staphylococci (positive) from streptococci (negative)
57
what bacteria can H2O2 kill?
staphylococci
58
used to differentiate Staphylococcus aureus from other less pathogenic staphylococci
coagulase test
59
can not only differentiate among microbial species, but also
serological testing (immunologic test)
60
strains with different antigens are called
serotypes, serovars, or biovars
60
strains with different antigens are called
serotypes, serovars, or biovars
61
different serological test/two blood antibody tests that may be used to detect HIV
ELISA and Western blotting

Microbiology and Parasitology (Lecture) (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions