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Flashcards in Bacteriology Deck (211)
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1

Gram-negative coccobacillus

non-motile

Acinetobacter spp.

2

Describe growth of Actinomadura madurae on quad plate containing casein, xanthine, tyrosine, and starch.

Growth on casein and starch, maybe tyrosine

3

Swelling of the neck beneath the left jaw with enlargement of cervical lymph nodes

GS of purulent material revealed non-acid fast branching GPR

Actinomyces israelii

Classic "Lumpy jaw" case

4

Beaded, branching anaerobic GPR, non-acid fast

Slow growing

Associated with head and neck abscesses

Actinomyces spp

5

In a urine culture, a small/pinpoint alpha-hemolytic, weakly pyrase pos GPCL

Sometimes see tetrads

Aerococcus urinae

6

Gastroenteritis

Oxidase positive GNR

Hydolyzes esculin

Aeromonas spp.

(or plesiomonas)

7

Anaerobic GNC

Acidaminococcus

Megasphaera

Veillonella

8

Anaerobic GPC

Peptostreptococcus

Finegoldia

Peptococcus (former peptostrep)

Peptoniphilus

Parvimonas

Anaerococcus

9

Pharyngitis in 10-30 year old with a rash

Slow growing, small, indistinct zone of β-hemolysis

GPR that may show rudimentary branching

Catalase-negative, urease negative

Shows CAMP-inhibition

Arcanobacterium haemolyticum

Penicillin-resistant (why you need to distinguish from Strep)

10

Non-hemolytic medusa head colony of the consistency of beaten egg whites

boxcar GPR

non-motile

FUCKING ANTHRAX. BAG THAT SHIT

Demonstrating either hemolysis or motility will rule it out

11

What are the forms of anthrax possible

Cutaneous, Inhalational (die), Gastrointestinal, Injection

12

Patient presents with acute food poisoning

Beta-hemolytic GPR in chains, some spores, catalase pos

Bacillus cereus

Can also cause serious eye infections after traumatic inoculation

13

Small anaerobic GNR resistant to vancomycin, colistin, kanamycin and bile

Bacterioides fragilis group

14

Only bacillus spp that cannot hydrolize lecithinase

B. subtilis

15

Grows on KV agar but no fluorescence

Produces black colonies on BBE

Resistant to penicillin

B. fragilis

16

GS with dog bone appearance

Bifidobacterium

Normal mouth and GI bug. Rarely causes disease.

17

Light pink colonies on chocolate

Motile, oxidase pos, non-fermenter

Oxidative utilization of maltose only

Rapid urease production and nitrate reduction

Bordatella bronchiseptica

18

GNCB produces small grey colonies surrounded by a zone of hemolysis on potato-blood-glycerol agar. Will grow on CA, not on BA.

Bordatella pertussis

19

Causative agent of Lyme Disease

  • Radiating rash (Erythema migrans)
  • Flu-like symptoms
  • Arthritis symptoms following bite

Diagnosed by rash and history of tick bite or serology

  • ELISA followed by Western to confirm

Borrelia burgdorferi

20

Diurnal fever in midwest patient + fatigue

Pos blood @ 9 days on choc

 

Brucella abortis

21

Brucella spp. GS

Small GNCB that appear gram positive due to retention of CV

22

Respiratory disease in CF patients

GNR, NLF grown on media with polymixin B and ticarcillin

drastically alters patient care and affects ability to receive lung transplant

Burkholderia cepacia

23

Cause  of glanders- aggressive pulmonary disease is most common presentation, inhalation or ingestion.  Overwhelming septicemia, draining abscesses.

Burkholderia mallei

24

Cause of meliodosis

Burkholderia pseudomallei

25

The presence of clue cells with gram variable coccobacilli is diagnostic of...

Bacterial vaginosis

Overgrowth of vaginal flora, including Mobiluncus, Prevotella, genital mycoplasmas, and Gardnerella vaginalis

26

C-rective protein is elevated in what circumstances?

Pediatric bacterial infections

27

Test to distinguish C. jejuni from C. coli

Hippurate hydrolysis

(C. jejuni is positive)

28

Gram stain: curved, S-shaped, or “gull-wing” gram negative rods

Media + Colony Morph

  • Selective media, elevated incubation temperature (42oC), and proper atmospheric conditions required for isolation
  • Colonies are flat, gray, irregular, and may be either dry or moist with glistening edges

Metabolism

  • Microaerophilic (require decreased O2)
  • Capnophilic (require increased CO2)
  • Nonfermentative and nonoxidative in metabolism
  • Oxidase positive

Campylobacter spp

29

Colony recovered after growth at 42oC was indoxyl acetate pos, as evidenced by a green spot on the indoxyl acetate disk.

Campylobacter upsaliensis

30

Capnophilic

Colony morphology --> gliding motility

Gram stain --> thin and fusiform (pointed ends)

Normal inhabitants of oral cavities of mammals

Capnocytophaga