BF MAMC

Question 1

Whats the dilution factor used for sperm motility

Answer 1

1: 20

Question 2

what’s the solution used to dilute sperm for motility

Answer 2

PBS - Phosphate buffer solution

Question 3

What does semen analysis includ

Answer 3

volume
color
viscosity
liquefaction
motility

Question 4

what’s the reference range for motility for semen

Answer 4

> 49%

Question 5

when is motility of a semen sample read

Answer 5

within 1 hour of collection

Question 6

Red cell diluent

Answer 6

saline

Question 7

Diluent that destroys red cells and enhances wbcs

Answer 7

3% glacial acetic acid

Question 8

for CSF analysis, what tubes are used to rule out bleeding

Answer 8

1 and 4

Question 9

When do we count tube 1 and 4

Answer 9

when the csf is xanthochromic or grossly bloody

Question 10

What tube do we count first for CSF

Answer 10

4

Question 11

what tube do we used to perform cell count

Answer 11

4 if clear

Question 12

When do we perform cell count on tube 4

Answer 12

when RBCs is >/10 or WBC >/4

Question 13

when do prepare cytospin (differential) for cell count

Answer 13

if WBCs is >4

Question 14

where is CSF produced

Answer 14

in choroid plexus

Question 15

how are the malignant cells in CSF called

Answer 15

choroid plexus

Question 16

what’s the function of CSF

Answer 16

provides nutrients to css
moves metabolic waste
mechanical barrier to cushion

Question 17

Whats require for CSF samples

Answer 17

total volume

clarity/clot

Question 18

what do we do we bloody CSF

Answer 18

spin down, if it does not clear is xathochromic

Question 19

When do we perform WBC count on normal CSF

Answer 19

when heme or Onco patient

Question 20

when do we perform cell count in tube 2 for CSF

Answer 20

leukemia/cancer

Question 21

when do we perform cell count in tube 3 for CSF

Answer 21

to rule out meningitis

Question 22

What does xanthochromsia indicates

Answer 22

traumatic tap, intracerebral or subarachnoid hemorrhage

Question 23

How do we perform a cell count in CSF

Answer 23

1 drop of albumin + 2 or 3 drops of CSF

Question 24

What cells can we see in CSF

Answer 24

Neutrophil, Lymph, Eso, baso, Mono macro

Question 25

a macro/mono with iron in the cytoplasm

Answer 25

hemosiderin

Question 26

characteristic of choroid plexus

Answer 26

``` clumping larger than normal dark color phagocytosis bledding ```

Question 27

What do we used to collect synovial fluid

Answer 27

a purple tap or a syringe with EDTA

Question 28

How do you lyse RBC in synovial fluid

Answer 28

hyaluronidase

Question 29

How do you eliminate viscosity of synovial

Answer 29

hyaluronidase, saline dilution or saline wash

Question 30

how do we check for crystal in synovial fluid

Answer 30

using a wet prep

Question 31

what are the two type of crystal in synovial fluid

Answer 31

MSU and CPPD

Question 32

what does MSU mean

Answer 32

Monosodium urate crystals

Question 33

what does cppd mean

Answer 33

Calcium Pyrophosphate Deposition

Question 34

what type of crystal is msu

Answer 34

gout

Question 35

what type of crystal is cppd

Answer 35

pseudogout

Question 36

what analyses we used to do cell count on synovial fluid

Answer 36

sysmex

Question 37

what do we do we normal results for synovial using sysmex

Answer 37

we release

Question 38

what do we do with

Answer 38

perform manual count

Question 39

how do we perform count in body fluids

Answer 39

using a cytospin

Question 40

what's a cytospin

Answer 40

concentrate cells in fluid

Question 41

how do we prepare a slide for synovial

Answer 41

cytospin + one drop

Question 42

what cells do we see in synovial

Answer 42

Neutrophil, Lymph, Eso, baso Mono macro synovial cell

Question 43

where do we see choroid plexus

Answer 43

in csf

Question 44

when do we see plasmacyte

Answer 44

all, specially black

Question 45

when do we see hemosideron

Answer 45

all fluids except synovial

Question 46

where do we see cholesterol crystal

Answer 46

in synovial fluid

Question 47

when do we see epithelial cell

Answer 47

in bla

Question 48

what are the different type of serous fluid

Answer 48

``` pericardial pleural ascites thoracentesis peritoneal ```

Question 49

when do we see mesothelia cell

Answer 49

in serous fluid

Question 50

a macrophage with a nucleus push to the side (like a half moon)

Answer 50

signet ring

Question 51

a macrophage with RBCs inside

Answer 51

erythro phagocytosis

Question 52

what abnormal macrophages can we see in serous fluid

Answer 52

hemosiderin meso signet ring erythro phagocytosis

Question 53

what cells are included in a serous cell count

Answer 53

neutro, lymp, eso, baso and macro

Question 54

are there monos in serous fluid

Answer 54

yes but they are called macro

Question 55

BLA

Answer 55

washing out lungs with saline

Question 56

is there mono in bla

Answer 56

nooooooooo

Question 57

characteristics of bronchial epic

Answer 57

light city cilia nucleus

Question 58

what happen when you centrifuge pseudochylous sample

Answer 58

they become clear

Question 59

what happen when you centrifuge chylous fluid

Answer 59

still milky

Question 60

Stain used for: Differential

Answer 60

Wright stain

Question 61

Stain used for: PCP

Answer 61

Silver Nitrate Stain

Question 62

Stain used for: Lipid Laden

Answer 62

Oil Red O

Question 63

Stain used for: Nasal smear and urine for eos

Answer 63

Hansel stain

Question 64

Stain used for: Urine for hemosiderin

Answer 64

Iron stain or Perls stain

Question 65

Stain used for: semen analysis

Answer 65

5% eosin Y and 10% nigrosin

Question 66

Sperm morphology

Answer 66

``` ovals doubles tail defects immature amorphous ```