BIO 111 Lab Quiz 3

Question 1

Visible light spectrum

Answer 1

Ultra- Gamma- X-ray- UV- IR- Radio

Question 2

Chlorophyll a peak absorption

Answer 2

430 nm

Question 3

Chlorophyll b peak absorption

Answer 3

450 nm

Question 4

Pigment that absorbs most visible light

Answer 4

Chlorophyll a

Question 5

Spectrophotometer

Answer 5

Helps us figure out which colors of light a sample absorbs by shining light on it and measuring what comes out.

Question 6

Most to least polar

Answer 6

Chlor b, Chlor a, Xanthophyll, Beta carotene

Question 7

Why most polar

Answer 7

Hydroxyl groups and the overall symmetry of the molecules. Presence of polar groups like (-OH) increases, while long hydrocarbon chains decrease.

Question 8

Paper chromatography separates mixtures based on differences in

Answer 8

Soulbilty

Question 9

DNA gyrase

Answer 9

Prevents supercoiling

Question 10

DNA polymerase I

Answer 10

Removes RNA primer

Question 11

Helicase

Answer 11

Breaks H bonds between bases

Question 12

DNA polymerase III

Answer 12

Elongates DNA strand from 3’OH end

Question 13

Primase

Answer 13

Places RNA primer

Question 14

SSBs

Answer 14

Stabilizes single-stranded DNA

Question 15

Binary fission

Answer 15

Bacteria perform asexual reproduction through

Question 16

Steps in cell cycle

Answer 16

G1 phase, S phase, G2 phase, M phase, Cytokinesis

Question 17

Anaphase

Answer 17

sister chromatids separate

Question 18

G2 phase

Answer 18

checkpoint to assess DNA replication

Question 19

Prophase

Answer 19

chromosomes condense

Question 20

Metaphase

Answer 20

chromosomes align in center of dividing cell

Question 21

Telophase

Answer 21

chromosomes elongate

Question 22

S phase

Answer 22

DNA replication occurs

Question 23

Null hypothesis

Answer 23

There is no difference; follow mendelian genetics

Question 24

Degrees of freedom

Answer 24

-1

Question 25

Reject hypothesis

Answer 25

Does not follow

Question 26

Expected value

Answer 26

Total x expected proportion (9:3:3:1)

Question 27

Buffer

Answer 27

Maintain stable pH, protect components

Question 28

What process used

Answer 28

Heating DNA

Question 29

Denaturation

Answer 29

Heat is used to break the hydrogen bonds that hold the two strands of the DNA together

Question 30

Annealing

Answer 30

Binding of primers to targeted DNA gene