BIO DAT Flashcards
Energy can be put into two categories (DAT favorite!):
a) Kinetic: energy of motion (e.g. blood flows)
b) Potential: energy that is stored (e.g. glycogen)
ATP allows
_____(endergonic/exergonic)_____ reactions to become
____(endergonic/exergonic)____.
Endergonic ——ATP—-> Exergonic
If you radio-label a S atom, what would it most likely end up in?
A. Carbohydrate
B. Protein
C. Lipid
B. Protein
Proteins contain C, H N, O, S
Which 3 amino acids are hydrophobic?
LIV “you need organic molecules to live”
Leucine
Isoleucine
Valine
Which 3 amino acids are hydrophilic?
LAG
- “if you don’t learn this shit you’re gonna lag getting into dental school”
Lysine (contains NH bond) Aspartic acid (aspartate) (contains OH) Glutamic acid (glutamate( (also contains OH)
How much free rotation do a polypeptide chain of amino acids have?
Very important DAT concept:
Very little
-Steric Hinderance cause the trans conformation to be more stable than cis
What 2 important biological molecules does sugar-phosphate “backbone” form the structural framework for?
DNA and RNA
“Know that for the DAT, you may thank me one day!”
What is the monomer of nucleic acids(many nucleotides) that connects the sugars of each nucleotide together?
Phosphodiester bonds
*nucleotides are linked together by phosphodiester bonds
What two organs contain the most mitochondria?
Heart > Kidneys»_space;» everything else
What 2 WBCs are loaded with lysosomes?
Macrophages and Neutrophils
-macrophages and neutrophils do a lot of phagocytosis
“Know that for the DAT, you may thank me one day!”
What are the 3 sections of the Golgi and what occurs at each?
Cis golgi —> molecules go in
Trans golgi —> molecules go outward
Main golgi —> molecules get processed
How and where are lysosomes formed?
lysosomes are formed by budding from the golgi complex
Budding: new individual splits off from existing one (hydra)
“A favorite DAT question”
What are many of the cells polysaccharides made by/from?
Golgi and glycosaminoglycans of the extracellular matrix
Part of which organelle is continuous (connected) with the nuclear envelope?
the Rough ER
“Know this for the DAT exam”
What is the difference between heterochromatin and euchromatin?
Heterochromatin = not actively transcribing because it’s Condensed (Dark stained cells in DNA)
Euchromatin = is lighter color and actively transcribing cells in DNA because it’s loosely packed
(both are near the nuclear)
What is the “mitotic poison” that messes up microtubules and halts mitosis?
colchicine
What are cilia and flagella composed of?
microtubules and motor proteins used for movement
What molecular motor enzymes can walk along microtubules and are responsible for the bending movements of cilia and flagella?
Dynein “walking” is responsible for the bending movements of cilia and flagella
and Kinesins
What is the pattern of the flagella and cilia?
“9+2” array
microtubules are arranged in 9 pairs (mm-mm-mm-mm-mm-mm-mm-mm-mm)
you can observe this using a transmission electron microsope
“A sure bet to appear on the DAT!!!”
What enzyme is used in the first step of glycolysis and what is the duty of this enzyme?
Kinase - catalyze the transfer of a phosphate group from a high energy molecule such as ATP.
“This is an important point”
What type of enzyme can make a glucose into a fructose?
Isomerase - changes a molecule into its isomer
What type of enzyme catalyzes rxns forming NADH and FADH2 from NAD+ and FAD+?
Dehydrogenase
“If you see NAD+, NADH, FAD, FADH2… you are most likely dealing with a hydrogenase”
“A must have name for the DAT exam”
What is the bottom line thing you need to understand about what acetyl CoA does for us?
the TCA cycle dismantles acetyl groups converting them into CO2 and H- and H+ into the ETC to produce ATP
“essential to know”
Acetyl CoA contains what functional group?
Thioester