Bio Exam ! Flashcards

(35 cards)

1
Q

DNA

A

double-stranded made of deoxyribonucleotides

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2
Q

Liter

A

l

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3
Q

Milliliter

A

ml 10^-3

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4
Q

Microliter

A

ul 10^-6

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5
Q

Concentration units

A

mM, X, mg/mL %, M, g/l

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6
Q

mRNA

A

Type of RNA that carries the genetic information from DNA to the ribosome, where proteins are synthesized

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7
Q

Pre-mRNA

A

Initial transcript before it becomes mRNA (contains both introns and exons)

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8
Q

tRNA

A

small RNA molecule that carries amino acids to ribosomes

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9
Q

Intron

A

non-coding sequence
(removed during the process of RNA)

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10
Q

Exons

A

coding sequence

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11
Q

rRNA

A

Single stranded made of ribonucleotides

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12
Q

spliceosome

A

responsible for removing introns into pre-mRNA

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13
Q

Ribosome

A

makes proteins by translating mRNA to amino acids

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14
Q

cDNA

A

lacks introns, basically a copy of mRNA

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15
Q

Reverse transcription

A

mRNA to cDNA

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16
Q

Reverse transcriptase

A

an enzyme that converts RNA into DNA

17
Q

Protein

A

structure that causes function to occur (amino acid)

18
Q

DNA Polymerase

A

creates new DNA

19
Q

RNA Polymerase

A

enzyme that transcribes DNA into RNA

20
Q

Polymerase Chain Reaction (PCR)

A

amplify DGK gene, allows us to isolate and copy specific sections of DNA

21
Q

Annealing

A

to bind 55-65

22
Q

Denaturing

A

to separate 95

23
Q

Extension

A

makes new DNA strands 72

24
Q

Polymorphism

A

2 or more of a variant of a specific DNA sequence

25
indel
(insertion/deletion) inserted or deleted from the DNA sequence
26
Sense & Synonymous Mutation
SNP mutation that does not change the amino acid
27
Nonsynonymous mutation
SNP mutation that does change the amino acid
28
Missense Mutation
changes an amino acid to another amino acid
29
Nonsense mutation
changes an amino acid to a stop codon
30
Transposable Element
"Reversable mutations"
31
primers
create new DNA synthesis
32
Plasmid
small, circular, double stranded DNA that has antibiotic resistance.
33
Bacterial Transformation
inserting DNA into bacteria
34
Constitutive promoter
promoter that is one all the time
35
Conditional promoter
promoter requires something to turn it on