Flashcards in BioChem Deck (153)
Transfer elecltrons from a donor to an acceptor
Transfer a functional group
Rearrange / isomerase a molecule
Add or remove atoms to or from a double bond
Form (C-O C-S C-N C-C) bonds with the hydrolysis of atp
Cleave bonds via the addition of water
Enzyme that uses Cu as a cofactor
Cytochrome c oxidase
Enzyme that uses Fe as cofactor
Heme proteins (hemoglobin and myoglobin) need Fe to bind O2
Enzyme that requires Mg as a cofactor
Enzyme that requires Se as a cofactor
Glutathione peroxidase- detoxifies hydrogen peroxide
Enzyme that requires Zn as a cofactor
Superoxide dismutase - binds the free radical of molecular oxygen
2 types of coenzymes
Co substrate - temporary association such as NAD+
Prosthetic - permanent association Heme
Ideal temp for enzyme activity
37 degrees C
Ideal pH for enzyme activity
4-8 with the exception of pepsin
Gastric proton pumps are on what type of cell
What variable stands for enzyme - substrate affinity?
/What shape is a Michaelis menten plot?
/What shape is a lineweaver Burke plot?
Resemble substrate - compete for the active site on an enzyme
No effect on vmax - increase Km
Lineweaver Burke x intercept
Y intercept lineweaver Burke
Slope lineweaver Burke plot
Non competitive inhibitor
Bind to E or ES complex not at the active site
Effect cannot be altered by increasing substrate concentration
Decrease in vmax and Km by the same factor - lineweaver Burke plot with and without inhibitor yields parallel lines
How can you inhibit metaloenzymes
Use metal cofactor - chelating the cofactor will inhibit enzyme activity
This can be used to stop lead poisoning through using edta which is a lead chelator (lead normally will bind heme rendering it useless)
VMax? Up or down
Km? Up or down
Reversible or not?
Similar lineweaver Burke plot to what type on inhibition?
Non competitive is similar but is reversible
Type of bond ?
Location of bond?
Positive or negative effect?
Not at the catalytic site
Induce conformational change to create a positive or negative impact
Utilize feedback inhibition where product of enzyme is a negative effector
What shape plot does a allosteric enzyme create