Biochem Basics Flashcards

0
Q

Any substance that resists a change in pH when protons are produced or consumed

A

Buffer

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1
Q

Negative logarithm of hydrogen ion concentration

A

pH

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2
Q

Chemical compound that has total net charge of zero

A

Zwitterion

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3
Q

pH at which the zwitterion is the predominant form of the chemical compound

A

Isoelectric pH

(pK before + pK after) / 2

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4
Q

Bond between a water molecule and another polar compound

A

Hydrogen bond

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5
Q

Bond between glucose and galactose in lactose

A

Glycosidic bond

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6
Q

Primary bond of glucosyl residues in glycogen

A

Alpha (1->4) glycosidic bind

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7
Q

Branching bond of glucosyl residues in glycogen

A

Alpha (1->6) glycosidic bond

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8
Q

Bond between glycerol and its fatty acid

A

Ester bonds

Dissolved by lipase

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9
Q

Bond between serine and alanine in a protein

A

Peptide bond

  • it’s a partial double bond
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10
Q

Bond between two cysteine residues in cystine

A

Disulfide bond

  • also bond in immunoglobulins
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11
Q

Bond between ribose and adenine in adenosine monophosphate

A

Beta N glycosidic bond

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12
Q

Bond between nucleotides on a DNA strand

A

Phosphodiester bond

-dissolved by phosphodiesterase

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13
Q

Bond between C-G and A-T

A

A-T. 2 hydrogen bonds

C-T. 3 hydrogen bonds

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14
Q

Equation used to calculate the concentration of a weak acid and its conjugate base

A

Henderson hasselbach equation

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15
Q

Equation describes how reaction velocity varies with substrate concentration

A

Michaelis-Menten

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16
Q

This is a double reciprocal plot used to calculate Km and Vmax, as well as to determine the mechanism of action of enzyme inhibitors

A

Lineweaver Burke plot

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17
Q

Explains how the free energy generated by the transport electrons through the ETC is used produce ATP from ADP+Pi

A

Mitchell Chemiosmosmotic Hypothesis

Explains oxidative phosphorylation

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18
Q

Accepts H+

A

Base

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19
Q

Donates protons

A

Acid

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20
Q

pKa below 7

21
Q

pKa above 7

22
Q

Carbohydrate, Protein, Lipid or Nucleic Acid?

Glycogen

A

Carbohydrate

23
Q

Carbohydrate, Protein, Lipid or Nucleic Acid?

cAMP

A

Nucleic acid

24
Carbohydrate, Protein, Lipid or Nucleic Acid? Collagen
Protein
25
Carbohydrate, Protein, Lipid or Nucleic Acid? Palmitate
Lipid
26
Carbohydrate, Protein, Lipid or Nucleic Acid? Inosine Monophosphate
Nucleic acid - it's the parent protein
27
Carbohydrate, Protein, Lipid or Nucleic Acid? Sorbitol from glucose reduction
Carbohydrate
28
Carbohydrate, Protein, Lipid or Nucleic Acid? Vitamin A
Lipid
29
Carbohydrate, Protein, Lipid or Nucleic Acid? Insulin
Protein
30
Substrate concentration at which the reaction velocity is equal to 1/2 Vmax
Michaelis constant
31
Maximal velocity of an enzyme catalyzed reaction
Vmax
32
The structure of the inhibitor resemblance the substrate
Competitive inhibition
33
Vmax is lowered by
Non competitive inhibitor
34
KM is not modified by the presence of
Non competitive inhibitor
35
Affinity of enzyme to the substrate is decreased by the presence of
Competitive inhibitor
36
The inhibitor and the substrate bind at different sites on the enzyme
Non competitive inhibition
37
Malathion's (organophosphate) effect on acetylcholinesterase
Non competitive inhibition
38
Simvastatin's effect on HMG-CoA reductase
Competitive inhibition
39
What is the formula for standard free energy
delta G = delta H - T delta S
40
Measure of energy available to do work
delta G or Gibbs free energy
41
Measure of randomness
delta S or entRopy
42
Measure of heat released or absorbed during a reaction
entHalpy Heat absorbed : endergonic : positive G Heat released: exergonic : negative G
43
Free energy change under standard conditions
Standard free energy Or delta G *
44
Two ways in which your cells produce ATP
Oxidative phosphorylation | Substrate level phosphorylation
45
Endergonic reaction
Positive G- heat is absorbed
46
Energy of the products is less than the energy of the reactants
Negative G
47
Exothermic reaction
Releases energy
48
Spontaneous reaction
Negative G
49
Rate of formation of products is the same as the rate of formation of the reactants
delta G is 0 Equilibrium