Biochem - Downstream Processing Flashcards
1
Q
- Concentration polarization can be reduced further by
A
- All of the above
2
Q
- A system which requires less solvent and produces a more concentrated extract phase, is desired with a
A
- Large distribution coefficients
3
Q
- The efficiency of cell disruption in a bead mill depends on the
A
- All of these
4
Q
- A common filter medium is the cloth filter generally made of
A
- All of these
5
Q
- An ion exchange resin is composed of
A
- All of these
6
Q
- Maintenance cost varies from
A
- 4-8% of the capital cost
7
Q
- The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between
A
- 15-30% by volume
8
Q
- Assuming the laminar flow across the filter, the rate of filtration (dVf/dt) can be expressed as a function of pressure drop Dp by the modified D’Arcy’s equation as
A
- (1/A)dVf/dt=Δpμ(L/K)dVf/dt(1/A)=μ(L/K)Δp
9
Q
- Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft22hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?
A
- 1.54 ft2
10
Q
- Selectivity can be expressed as
A
- Ratio of the distribution coefficients in different solute
11
Q
- The disk centrifuge is the type of centrifuge used most often for bio separations due to its
A
- Continuous operation
12
Q
- The effectiveness of a solvent can be measured by the
A
- Both (a) and (b)
13
Q
- The stage wise operation of adsorption is called
A
- Conventional adsorption
14
Q
- Which of the following is not the physical method for the cells rupturing?
A
- Enzymatic digestion
15
Q
- Conventional adsorption is a
A
- Reversible process
16
Q
- If the solute passes through membrane freely, the the rejection coefficient (s) is
A
- 0
17
Q
- If the membrane rejects all solute, the rejection coefficient (s) is
A
- 1
18
Q
- In antibiotic manufacturing processes, the fermentation time ranges from
A
- 2-4 weeks
19
Q
- The conventional filtration involves the separation of large particles generally
A
- dp>5μm
20
Q
- Which of the operation does not come under upstream processing?
A
- Effluent treatment
21
Q
- For cost analysis in a fermenter system, the depreciation cost varies from
A
- 6-10% of the capital cost
22
Q
- Micro filtration refers to the separation of suspended material such as bacteria by using a membrane with spore sizes of
A
- 0.02 to 10μm
23
Q
- Start up expenses in a fermentation industry varies from
A
- 5-10% of the capital cost
24
Q
- The solvent flux across the membrane can be expressed as
A
- J = Lp(Δp - σΔπ)
25
25. Which of the following is considered as a pretreatment to the biological feeds?
25. All of the above
26
26. For the extractions operation, the selectivity should be
26. 1
27
27. The purity of a solute collected between two times t1 and t2 during chromatographic separation can be calculated as
27. Amount of solute eluted / amount of impurity eluted
28
28. Chromatography is based on the
28. Different rate of movement of the solute in the column
29
29. The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)
29. Y = YmaxX/(KL+X)
30
30. A typical tubular centrifuge has a bowl of 2 to 5 inch in diameter and 9 to 30 inch in height with a maximum rotating speed of
30. 15,000 to 50,000 rpm
