Biochem Lecture 1

Question 1

Weak Acid Dissociation Equation

Answer 1

HA↔H ᐩ+ A﹣

Question 2

Buffer

Answer 2

Weak Acid ↔️ Hᐩ + Conjugate Base

Question 3

Keq

Answer 3

The equilibrium constant for a reversible chemical rxn. It depicts the concentration
When equilibrium is reached and which direction is favored.

Question 4

Keq Formula.

Products and Reactants are located where in the formula? Denominator and Numerator.

Answer 4

   	  [ H A ]

Question 5

↑ Keq

Answer 5

Strong Acid

Question 6

↓ Keq

Answer 6

Weak Acid

Question 7

products > reactants, what would be the Keq in reference to 1?

Answer 7

Keq> 1

Question 8

When Keq>1, which direction is favored? and whats the relationship between products to reactants?

Answer 8

Products > reactants, Reverse direction is favored.

Question 9

Products < 1, what would be the Keq in reference to 1?

Answer 9

Keq <1

Question 10

Keq <1, which direction is favored? What the relationship between products to reactants?

Answer 10

The forward direction is favored. Products < Reactants.

Question 11

What is pK in reference to Keq?

Answer 11

it’s the log inverse of Keq

pk = - log ( Keq)

Question 12

↓pk

Answer 12

Strong Acid

Question 13

↑pk

Answer 13

Weak Acid

Question 14

What is the best buffering range?

Answer 14

When pH = Pk,

Base = Acid

Question 15

How to find pH if given H+?

Answer 15

pH = - log [ H+ ]

Question 16

How to find pOH?

Answer 16

pOH= -log [OH-]

Question 17

What is physiological pH?

Answer 17

7.35-7.45

Question 18

Acid Bicarbonate buffer

Answer 18

CO2 + H20 — H2CO3—HCO3 + H

Question 19

High CO2, will result in a what kind of pH?

Answer 19

Low pH due to ↑ H +

Question 20

Low HCO3-, will result in what kind of pH?

Answer 20

Low pH due to free roaming ↑ H +

Question 21

How to find the PI of a Neutral Amino Acid?

Answer 21

                   2

Question 22

How to find the PI of a Acidic Amino Acid

Answer 22

                   2

Question 23

How to find the PI of a Basic Amino Acid?

Answer 23

                 2

Question 24

PI> pH

Answer 24

Positive charge

Question 25

PI< pH

Answer 25

Negative

Question 26

Henderson-hasselbalch equation

Answer 26

Relationship between pH of a solution and relative amounts of individual conjugate base and acid pairs pH=pk+log [Conjugate Base] /// [Conjugate acid]

Question 27

Log (1) =

Answer 27

0

Question 28

Log (10)

Answer 28

1

Question 29

Log (100)

Answer 29

2

Question 30

Log (1000)

Answer 30

3

Question 31

Hydrophilic AA traits

Answer 31

Acidic, Basic, Polar

Question 32

Name the Hydrophilic AA

Answer 32

Aspartate, Glutamate, Lysine, Arginine, Histidine, | Serine, Threonine, Cysteine, Tyrosine, Asparaginine, Gluatamine

Question 33

Basic/ polar AA

Answer 33

Lysine, Arginine, Histidine K, R, H Lys, Arg, His

Question 34

Acidic/polar AA

Answer 34

Glutamate Glu, E | Aspartate Asp, D

Question 35

Polar AA

Answer 35

``` Serine S, Ser Threonine, Thr, T Cysteine, Cys, C Asparganine, Asn, N Glutamine, Gln, Q Tyrosine, Tyr, Y ```

Question 36

Hydrophobic/ Nonpolar AA

Answer 36

``` Alanine, Ala Glycine, Gly Valine, Val Isoleucine, lle, I Leucine, Leu, L Phenylalanine, Phe, F Tryptophan, Trp, W Methionine Met, M Proline, Pro, P ```

Question 37

Unique AA

Answer 37

Glycine, Gly, G | Proline, Pro, P

Question 38

Sulfur containing AA

Answer 38

Cysteine, Cys, C | Methionine, Met, M

Question 39

How many codons are there?

Answer 39

64

Question 40

Start Codon

Answer 40

AUG which is encoded by a Met.

Question 41

Stop Codon

Answer 41

UAA UAG UGA

Question 42

Stop Codon UAG, encodes for which rare AA?

Answer 42

Pyrrolysine.. Remember : U ARE GREAT, PERIOD.

Question 43

Stop Codon UGA, encodes for which rare AA?

Answer 43

Selenocysteine.. Remember: | U GET ANOTHER Selena!

Question 44

How are peptide bonds made? What is released?

Answer 44

Carboxyl group on one amino acid combine with the Amino group on another amino acid. H20 is released.

Question 45

Amino Acid in a basic solution will

Answer 45

give it’s + from NH3+----> resulting in a NEGATIVE charged AA

Question 46

Amino Acid in a acidic solution will

Answer 46

accept a H+ proton to its COO- --> resulting in a POSITIVE AA.

Question 47

pH < Pka

Answer 47

COOH (protonated)

Question 48

pH> Pka

Answer 48

COO- (deprotonated)