Biochemistry - Lab Techniques Flashcards Preview

Boards Prep > Biochemistry - Lab Techniques > Flashcards

Flashcards in Biochemistry - Lab Techniques Deck (11)
Loading flashcards...
0

Southern Blot

DNA
DNA cleaved into smaller pieces--> electrophoresis and filtered --> DNA is radio labeled --> DNA visualized

1

Northern Blot

RNA
RNA is enzymatically cleaved--> electrophoresis and filtered --> radio labeled --> RNA visualized

Indicated the level of mRNA and gene expression

2

Western Blot

Protein
Proteins separated and electrophoresis --> labeled with antibody

* used to verify HIV dx after POSITIVE ELISA

3

SouthWestern Blot

identify DNA binding Proteins (transcription factors) using oligonucleotide probes

4

Microarrays

Used to identify gene expression, SNPs, CNV, genetic testing etc

Profiles the DNA by complementary binding with known DNA/RNA sequences

5

ELISA

Detect the presence of an antibody or an antigen
Direct: antibody used to detect presence of an antigen, antibody coupled to a color generating enzyme
Indirect: Antibody or antigen detected, then color generating enzyme used to detect the antigen-antibody complex

6

Karyotyping

Metaphase Chromosomes are detected, stained, and ordered based on various factors
- dx CHROMOSOMAL ABNORMALITIES

7

Floresence in Situ

Complementary binding with DNA/RNA probes to a specific gene of interest
Used for localization of the genes and direct visualization of anomalies

8

Cloning

mRNA --> cDNA via reverse trancscriptase --> inserted into plasmid with antibiotic resistance gene --> antibiotic resistance Bacteria created

9

Cre-lox

Can inducible modify the expression of genes at different points in development

10

RNA interference

dsRNA is synthesized that is complementary to the mRNA of interest. dsRNA is inserted into the human cells, and the dsRNA separates and combines with the host cell mRNA promoting degradation.
- to knock out gene expression