Flashcards in Biochemistry - Lab Techniques Deck (11)
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Southern Blot
DNA
DNA cleaved into smaller pieces--> electrophoresis and filtered --> DNA is radio labeled --> DNA visualized
1
Northern Blot
RNA
RNA is enzymatically cleaved--> electrophoresis and filtered --> radio labeled --> RNA visualized
Indicated the level of mRNA and gene expression
2
Western Blot
Protein
Proteins separated and electrophoresis --> labeled with antibody
* used to verify HIV dx after POSITIVE ELISA
3
SouthWestern Blot
identify DNA binding Proteins (transcription factors) using oligonucleotide probes
4
Microarrays
Used to identify gene expression, SNPs, CNV, genetic testing etc
Profiles the DNA by complementary binding with known DNA/RNA sequences
5
ELISA
Detect the presence of an antibody or an antigen
Direct: antibody used to detect presence of an antigen, antibody coupled to a color generating enzyme
Indirect: Antibody or antigen detected, then color generating enzyme used to detect the antigen-antibody complex
6
Karyotyping
Metaphase Chromosomes are detected, stained, and ordered based on various factors
- dx CHROMOSOMAL ABNORMALITIES
7
Floresence in Situ
Complementary binding with DNA/RNA probes to a specific gene of interest
Used for localization of the genes and direct visualization of anomalies
8
Cloning
mRNA --> cDNA via reverse trancscriptase --> inserted into plasmid with antibiotic resistance gene --> antibiotic resistance Bacteria created
9
Cre-lox
Can inducible modify the expression of genes at different points in development
10
