Bioconjugation Flashcards
(13 cards)
What is an antibody drug conjugate?
A pharmaceutical known as a bioconjate
At least one molecule that makes up the ADC is a biological molecule
Used for targeted therapy
Outline the structure of an antibody
Made up of amino acid sequence and has a Mw of 150KDa
Y shape
Contains antigen binding site.
What are the different portions of an antibody?
Fc, Fab2, Fab
Disulphide bridge
Lysine groups
Light and heavy chains
Outline structure of ADC
Site specific delivery of cytotoxic drugs
Stable linker
Ouline structure of the linker
Cleavable
Stability in circulation long term storage
Active release
Disulphide
Hydrazone
Peptide/protease
What are linker properties?
Spacer
Flexible
Cleavable
Stable
Non toxic
Inert
Photoactivable
Define homo bifunctional linkers
Linkers with the same reactive group
Define hetero bifunctional linkers
Linkers with different reactive groups
What are purification techniques for Bioconjugates?
Size exclusion chromatography SEC
Ion exchange chromatography IEC
Hydrophobic interactive chromatography HIC
Affinity chromatography AC
Outline SEC
Large particles cannot enter gel and are excluded. They have less volume to traverse and elute sooner.
Small particles can enter gel and have more volume to traverse. They elute later
Outline IEC
Positively charged proteins bind to negatively charged beads
Negatively charged proteins flow through
Outline HIC
The more hydrophobic groups that are attached the more readily the bioconjugate is retained
What are the characterisations of ADCs?
Separate antibody conjugates with different drug antibody ratio
Gives us information about the structure of the Bioconjugate
Antibody will have several accessible lysine residues
