BIOL4004 CB Part B Name 3 factors that affect the ability of enzymes to catalyse a reaction. Explain each how this happens (2016)

Question 1

Factors that effect enzymes to catalyse a reaction:

Answer 1

1.
Temperature

2.
pH

3.
Presence of inhibitors

4.
The concentration of enzyme/ substrate.

Question 2

Explain the pH affects of enzyme to catalyse a reaction:

Answer 2

As temperature increases so does the reaction rate - collision theory, but eventually denatures enzymes by breaking H bonds

Question 3

Explain the temperature affects of enzyme to catalyse a reaction:

Answer 3

pH - There is an optimal pH. Either side of this may be changes in the charge of the active site, affecting substrate binding and at extremes of pH, H and ionic bonds holding the enzyme and protein in shape can be broken (enzyme denatured).

Question 4

Explain the inhibitors affects of enzyme to catalyse a reaction:

Answer 4

Presence of inhibitors - either competitive and non-competitive - reduces substrate binding and therefore reduces reaction rate.

Question 5

Explain the concentration affects of enzyme to catalyse a reaction:

Answer 5

[4]
Or concentration of enzyme/ substrate. Low concentration of enzyme to substrate will result in slow reaction - enzymes spend time bound to substrate, so can only work a set speed. Add more enzyme, more active site so more reactions.